File:The-cation-channel-TRPA1-tunes-mosquito-thermotaxis-to-host-temperatures-elife-11750-fig3-figsupp2.jpg

English: (A) Genomic organization and targeted mutagenesis of AaegGr19. Coding (black) exons are shown to scale, introns are denoted by connecting lines (not to scale), and alternative splicing is denoted by dashed lines (vectorbase.org). The AaegGr19 ZFN targets exon 3 (green arrow). An insertion cassette (shown to scale), including the Ae. aegypti polyubiquitin (polyUB) promoter driving expression of Discosoma sp. red fluorescent protein (DsRed) and an SV40 polyadenylation signal, is integrated via homology-directed repair. (B) Representative bright field (left) and fluorescence (right) images of wild-type and AaegGr19-/- female pupae marked with ubiquitous expression of DsRed Scale bars: 0.5 mm. The wild-type bright-field image is duplicated from (Figure 3A). (C) Percent of mosquitoes of indicated genotypes on Peltier during seconds 90–180 of stimuli of indicated temperature (mean ± s.e.m., n = 6–9 trials per genotype). Neither AaegGr19-/- nor AaegGr19+/- were significantly different from wild-type at any stimulus temperature (repeated measures one-way ANOVA with Bonferroni correction).