File:The-cation-channel-TRPA1-tunes-mosquito-thermotaxis-to-host-temperatures-elife-11750-fig1.jpg

English: (A) Schematic of heat-seeking assay enclosure (30 × 30 × 30 cm). (B) Representative experimental image showing mosquitoes detected on and near the Peltier (red square). (C) Typical skin and core temperatures of Ae. aegypti hosts, humans and chickens (Richards, 1971; Yao, et al., 2008. (D–F) Heat-seeking behavior measured for a range of stimuli from 26 to 60°C (n = 6 trials per condition). Peltier temperature measured by thermocouple (D, top trace, mean in red, s.e.m. in gray) and percent of mosquitoes on Peltier (D, bottom trace, mean in black, s.e.m. in gray). We note that variance in both traces is low, making s.e.m. traces difficult to see. (E) Percent mosquitoes on Peltier during seconds 90–180 of each stimulus period in (D). Each replicate is indicated by a dot, and mean by a line. Arrowheads indicate significant differences (p < 0.05) from the second presentation of the 40°C stimulus or from 26°C (repeated measures one-way ANOVA with Bonferroni correction). (F) Heat maps showing mean mosquito occupancy on the Peltier (red square) and surrounding area, during seconds 90–180 of each stimulus period in (D). Bold borders indicate stimuli with responses significantly different from 26°C stimulus (top row) or 40°C stimulus (bottom row) in (E) (p < 0.05; repeated-measures ANOVA with Bonferroni correction).