File:TTL pNOB MMEAA graphs.png
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English: Michaelis-Menten Esterase Activity Assay of Thermoanaerobacter thermohydrosulfuricus lipase with 4-nitrophenyl butyrate (pNOB). Assays were conducted with 1.5 µM of lipase in PBS with pNOB concentration ranging from 0.0365 - 0.11 µM. The rate of change of absorbance was monitored at 410 nm for 1 min. A) Wild-type TTL lipase chromogenic assay with initial rate as a function of substrate concentration at 25 B) Wild-type TTL lipase chromogenic assay with initial rate as a function of substrate concentration at 60 ℃ C) TTL lipase chromogenic assay with initial rate as a function of substrate concentration, with norleucine introduced by residue specifically by SPI at 25 ℃ D) TTL lipase chromogenic assay with initial rate as a function of substrate concentration, with norleucine introduced by residue |
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| Source | Own work |
| Author | Prefont5 |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 01:27, 12 October 2023 | | 2,492 × 1,934 (266 KB) | Prefont5 (talk | contribs) | Uploaded own work with UploadWizard |
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