File:Stain and Reagents for Clinical Parasitology Laboratory.jpg
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[edit]DescriptionStain and Reagents for Clinical Parasitology Laboratory.jpg |
English: Here's a list of commonly used stains and reagents in a clinical parasitology laboratory:
Wet Mounts: Saline wet mount: Used to observe motile parasites and trophozoites. Simply uses a drop of saline with the specimen. Iodine wet mount: A drop of Lugol's iodine can be added to highlight the internal structures of parasites, such as nuclei and cyst walls. Permanent Stains: Trichrome stain: Commonly used for intestinal protozoa, especially amoebae and flagellates. Iron hematoxylin stain: Another stain used for intestinal protozoa. Modified acid-fast stain: Specifically designed for coccidia like Cryptosporidium and Isospora. Giemsa stain: Often used for blood parasites like Plasmodium (malaria) and Trypanosoma. Concentration Methods: Formalin-ethyl acetate concentration technique: Used to concentrate parasites from stool specimens. Zinc sulfate floatation: Another concentration technique. Other Reagents: Formalin: Used as a preservative for stool samples. Buffered glycerol: Used in the preparation of permanent slides. Potassium hydroxide (KOH): Can help clear specimens. Methylene blue: Can be used as a counterstain or to highlight certain structures. Phenol: Sometimes used in the preparation of certain stains or as a component of preservatives. Special Stains and Techniques: Auramine-rhodamine stain: Used for the detection of Cryptosporidium oocysts and other coccidia in stool. Silver stains (e.g., Warthin-Starry and Gomori's methenamine silver): For the visualization of certain tissue parasites. Serological tests: Some parasites can be detected by their antigens or the antibodies they induce in the host. Reagents for Fixation: Polyvinyl alcohol (PVA) fixative: Commonly used for preserving stool samples when subsequent staining is anticipated. Schaudinn's fixative: Another fixative that can be used for stool specimens. |
Date | |
Source | Own work |
Author | Ajay Kumar Chaurasiya |
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current | 14:37, 14 October 2023 | 3,264 × 2,448 (2.17 MB) | Ajay Kumar Chaurasiya (talk | contribs) | Uploaded own work with UploadWizard |
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Date and time of data generation | 13:58, 7 August 2015 |
Lens focal length | 3.7 mm |
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Orientation | Normal |
Horizontal resolution | 72 dpi |
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File change date and time | 13:58, 7 August 2015 |
Y and C positioning | Centered |
Exposure Program | Aperture priority |
Exif version | 2.2 |
Date and time of digitizing | 13:58, 7 August 2015 |
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