File:Spindle with Four Poles (8744518858).jpg

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Spindle_with_Four_Poles_(8744518858).jpg(448 × 446 pixels, file size: 93 KB, MIME type: image/jpeg)

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Summary

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Description The photograph in figure 1 shows a multipolar mitotic spindle with four poles rather than the two poles which would be expected in a normal cell. The tubulin in figure 1 was stained red using Spectrum red and indirect immunofluorescence. The DNA was detected by DAPI and was blue. The DNA is being pulled 4 ways. The cell was treated with carbon nanotubes. The nanotubes in figure 1 were imaged using differential interference contrast and are black. In the figure, the DNA, tubulin, and nanotubes images are merged. The nanotubes can be seen in the nucleus in association with microtubules and the DNA. Yellow arrows indicate the location of the nanotubes in the merged image. Serial optical sections at 0.1 micron intervals using confocal microscopy confirmed the location of the nanotubes in the nuclear DNA and the tubulin including the microtubules of the mitotic spindle.
Date
Source Spindle with Four Poles
Author National Institute for Occupational Safety and Health (NIOSH) from USA

Licensing

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Public domain
This image is a work of the National Institute for Occupational Safety and Health, part of the Centers for Disease Control and Prevention in the United States Department of Health and Human Services, taken or made as part of an employee's official duties. As a work of the U.S. federal government, the image is in the public domain.

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This image was originally posted to Flickr by NIOSH at https://www.flickr.com/photos/25069384@N03/8744518858. It was reviewed on 26 April 2015 by FlickreviewR and was confirmed to be licensed under the terms of the United States Government Work.

26 April 2015

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Date/TimeThumbnailDimensionsUserComment
current21:19, 26 April 2015Thumbnail for version as of 21:19, 26 April 2015448 × 446 (93 KB)Emily Temple-Wood (NIOSH) (talk | contribs)Transferred from Flickr via Flickr2Commons

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