File:Simple chromatography column cooling.jpg

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Simple chromatography column cooling

Summary

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Description
English: Any non-jacketed chromatography column can be kept cool using this low tech solution.
Date
Source Own work
Author Mjeltsch
Camera location60° 11′ 25.3″ N, 24° 54′ 19.98″ E Kartographer map based on OpenStreetMap.View this and other nearby images on: OpenStreetMapinfo

The column is connected to an Äkta Avant, which has a refrigerated fraction collector, but most of the columns designed for it are not the cooling (jacketed) type. This is a low tech solution, which we used when loading large sample volumes directly to the column. Loading large sample volumes takes a long time and we do not want the protein to be at room temperature for many hours. When the bottle is filled with ice water, it keeps the column cold for about 3-6 hours (depending on the ambient temperature).

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Date/TimeThumbnailDimensionsUserComment
current18:14, 21 March 2019Thumbnail for version as of 18:14, 21 March 20192,976 × 3,968 (1.16 MB)Mjeltsch (talk | contribs)User created page with UploadWizard

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