File:Sensor Development and Zika Virus Detection 160816-A-AB123-002.jpg

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English: The rapid design, assembly and validation of paper-based biomolecular sensors for the

portable detection of Zika virus is shown here. Using sequence information from online databases, primers for isothermal RNA amplification and toehold switch-based RNA sensors are designed in silico using algorithms. Sensors are embedded into paper discs and freeze-dried along with a cell-free transcription and translation system, to be deployed in the field as colorimetric diagnostics. In the diagnostic test, viral RNA is extracted, isothermally amplified, and used to rehydrate the freeze-dried sensors, resulting in a color change from yellow to purple in the presence of Zika RNA. Strain differentiation at single-base resolution is provided by a CRISPR-based module which connects to a secondary paper-based test that also provides colorimetric output.

Unit: Defense Threat Reduction Agency's Chemical and Biological Technologies Department
DVIDS Tags: Collins; James Collins; Dengue; DEFENSE THREAT REDUCTION AGENCY; DTRA; DTRA CB; MIT; CB; JSTO; Chem Bio; Joint Science and Technology Office; Chem/Bio; diagnostic; diagnostic equipment; Massachusetts Institute of Technology; Elashvili; DTRA-CB; Defense Threat Reduction Agecny; Defense Threat Reduction Agency Chemical and Biological Technologies Department; Diagnostic Devices; Chemical and Biolgoical Technologies; ZIKA; Zika Virus; Defense Threat Reduction Agency CB; Defense Threat Reduction Agency Chemical & Biological Technologies Department; Joint Science and Technology Department; Joint Science and Technologies; DTRA-CB/JSTO; Zika Detection; James J. Collins; Defense Threat Reduction Agency Chemical andBiological Technologies Department; American Zika; African Zika; CRISPR; Elshvili; LacZ
Date Taken on 16 August 2016
Source https://www.dvidshub.net/image/2795487/sensor-development-and-zika-virus-detection
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Location
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FORT BELVOIR, VA, US
VIRIN
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This image was released by the United States Army with the ID 160816-A-AB123-002 (next).
This tag does not indicate the copyright status of the attached work. A normal copyright tag is still required. See Commons:Licensing.

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Posted
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16 August 2016, 12:07
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archive copy at the Wayback Machine

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Public domain
This file is a work of a U.S. Army soldier or employee, taken or made as part of that person's official duties. As a work of the U.S. federal government, it is in the public domain in the United States.

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current11:01, 21 February 2017Thumbnail for version as of 11:01, 21 February 20172,237 × 2,208 (406 KB)Fæ (talk | contribs)DVIDS 4 megapixel image from https://www.dvidshub.net/image/2795487/sensor-development-and-zika-virus-detection. Collins; James Collins; Dengue; DEFENSE THREAT REDUCTION AGENCY. Part of User:Fæ/Project list/DoD

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