File:RsEGFP2-enables-fast-RESOLFT-nanoscopy-of-living-cells-elife00248fs006.jpg
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[edit]DescriptionRsEGFP2-enables-fast-RESOLFT-nanoscopy-of-living-cells-elife00248fs006.jpg |
English: (A),(B) Typical examples. Shown are raw images of cells expressing Keratin19-rsEGFP2 taken on a RESOLFT Quad P microscope (Abberior Instruments GmbH, Göttingen, Germany) with a pixel dwell time similar as in Figure 3D,E. Imaging conditions: (on: 405 nm, 8 kW/cm², 2 µs; off: 488 nm, 68 kW/cm², 61 µs; read-out: 488 nm, 200 kW/cm², 7 µs; pixel size: 40 × 40 nm). From left to right: confocal raw image and corresponding raw RESOLFT image. The graphs show averaged line profiles across the indicated filaments within the respective boxes. The line profiles used for averaging were taken equidistant (40 nm) along the whole respective indicated area. Scale bar: 1 µm. |
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Source | Image file from Grotjohann T, Testa I, Reuss M, Brakemann T, Eggeling C, Hell S, Jakobs S (2012). "rsEGFP2 enables fast RESOLFT nanoscopy of living cells". eLife. DOI:10.7554/eLife.00248. PMID 23330067. PMC: 3534202. | |
Author | Grotjohann T, Testa I, Reuss M, Brakemann T, Eggeling C, Hell S, Jakobs S | |
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![]() ![]() This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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current | 18:45, 16 October 2014 | ![]() | 586 × 420 (79 KB) | Recitation-bot (talk | contribs) | Automatic upload of media from: doi:10.7554/eLife.00248 |
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