File:R-Ras-regulates-β1-integrin-trafficking-via-effects-on-membrane-ruffling-and-endocytosis-1471-2121-11-14-S7.ogv
R-Ras-regulates-β1-integrin-trafficking-via-effects-on-membrane-ruffling-and-endocytosis-1471-2121-11-14-S7.ogv (Ogg Theora video file, length 5.7 s, 222 × 222 pixels, 202 kbps, file size: 142 KB)
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[edit]DescriptionR-Ras-regulates-β1-integrin-trafficking-via-effects-on-membrane-ruffling-and-endocytosis-1471-2121-11-14-S7.ogv |
English: GFP-R-Ras is endocytosed to lysosomes via a non-caveolin-1 mediated
pathway. (A-C) Shown are enlarged images of unfixed cells transfected with GFP-R-Ras constructs that were incubated with 1 μM Lysotracker Red DND-99 for 20 mins followed by washout. All Lysotracker-positive compartments larger than 1.2 μm contained R-Ras (n = 7 GFP-R-Ras(wt) cells, n = 8 GFP-R-Ras(38V) cells). There were numerous, smaller compartments in all cell types with no correlation between GFP-R-Ras and Lysotracker. (D-F) Transfected cells were fixed and stained for phospho-specific (Tyr-14) caveolin-1, but the lack of correspondence with GFP-R-Ras suggests that GFP-R-Ras is endocytosed separate from caveolin-1. Scale bar is 20 μm. |
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Date | |||
Source | Conklin M, Ada-Nguema A, Parsons M, Riching K, Keely P (2010). "R-Ras regulates β1-integrin trafficking via effects on membrane ruffling and endocytosis". BMC Cell Biology. DOI:10.1186/1471-2121-11-14. PMID 20167113. PMC: 2830936. | ||
Author | Conklin M, Ada-Nguema A, Parsons M, Riching K, Keely P | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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current | 01:43, 19 November 2012 | 5.7 s, 222 × 222 (142 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 7 |
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Author | Conklin M, Ada-Nguema A, Parsons M, Riching K, Keely P |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | GFP-R-Ras is endocytosed to lysosomes via a non-caveolin-1 mediated
pathway. (A-C) Shown are enlarged images of unfixed cells transfected with GFP-R-Ras constructs that were incubated with 1 ?M Lysotracker Red DND-99 for 20 mins followed by washout. All Lysotracker-positive compartments larger than 1.2 ?m contained R-Ras (n |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010 |