File:Proximal VE and ExE are sufficient to induce PGCs in distal epiblast.png
Jump to navigation
Jump to search
Size of this preview: 274 × 598 pixels. Other resolutions: 110 × 240 pixels | 220 × 480 pixels | 352 × 768 pixels | 1,142 × 2,493 pixels.
Original file (1,142 × 2,493 pixels, file size: 2.96 MB, MIME type: image/png)
Captions
Captions
Add a one-line explanation of what this file represents
Summary
[edit]
| Description | Fig. 4. Proximal VE and ExE are sufficient to induce PGCs in distal epiblast. (A) Schematic of the experimental design. E6.75–E7.0 Stella:gfp embryos were isolated and either the VE was isolated whole (cup shaped), the proximal region containing the PGC precursor cluster surgically removed and the VE "filled" with the ExE and distal epiblast (-cluster); or the embryo was cut in three parts and the part containing the PGC precursor cluster discarded (-cluster -proximal VE). (B-G) Explants derived from E6.75–E7.0 whole (B, C), -cluster (D, E) and -cluster -proximal VE (F, G) immunostained to detect Stella(GFP) (B, D, F), followed by staining to detect alkaline phosphatase-activity (C, E, G). Arrows show same PGCs in the different panels. (H-J) Sagittal section showing double LacZ and alkaline phosphatase-activity staining in E6.75 wild type (H), ROSA26:LacZ (I) and -cluster (J) explants cultured for 3 days. In -cluster explants, the ExE and VE were from wild type embryos and the distal epiblast was from a ROSA26:LacZ embryos. Arrows show the PGC cluster in each explant; the VE is also indicated. Scale bar is 200 μm. |
| Date | |
| Source | https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-7-140 de Sousa Lopes, S.M.C., Hayashi, K. & Surani, M.A. Proximal visceral endoderm and extraembryonic ectoderm regulate the formation of primordial germ cell precursors. BMC Dev Biol 7, 140 (2007). https://doi.org/10.1186/1471-213X-7-140 |
| Author | de Sousa Lopes, S.M.C., Hayashi, K. & Surani, M.A. |
|
This file, which was originally posted to an external website, has not yet been reviewed by an administrator or reviewer to confirm that the above license is valid. See Category:License review needed for further instructions.
|
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Licensing
[edit]
This file is licensed under the Creative Commons Attribution 2.0 Generic license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
File history
Click on a date/time to view the file as it appeared at that time.
| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 17:34, 6 September 2024 | | 1,142 × 2,493 (2.96 MB) | Rasbak (talk | contribs) | {{Information |description=Fig. 4. Proximal VE and ExE are sufficient to induce PGCs in distal epiblast. (A) Schematic of the experimental design. E6.75–E7.0 Stella:gfp embryos were isolated and either the VE was isolated whole (cup shaped), the proximal region containing the PGC precursor cluster surgically removed and the VE "filled" with the ExE and distal epiblast (-cluster); or the embryo was cut in three parts and the part containing the PGC precursor cluster discarded (-cluster -proxim... |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
