File:Principle of Light Sheet Fluorescence Microscopy (LSFM).jpg
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[edit]DescriptionPrinciple of Light Sheet Fluorescence Microscopy (LSFM).jpg |
English: Light sheet fluorescence microscopy (LSFM) splits fluorescence excitation and detection into two separate light paths, with the axis of illumination perpendicular to the detection axis. That means you illuminate a single thin section of the sample at one time, generating an inherent optical section by exciting only fluorescence from the in-focus plane. No pinhole or image processing is required. Light from the in-focus plane is collected on the pixels of a camera, rather than pixel by pixel as, for example, in confocal or other laser scanning microscopes. Parallelization of the image collection on a camera-based detector lets you collect images faster and with less excitation light than you would with many other microscope techniques.
www.zeiss.com/lightsheet |
Date | |
Source | https://www.flickr.com/photos/zeissmicro/8523991917/ |
Author | ZEISS Microscopy |
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[edit]This image was originally posted to Flickr. Its license was verified as "cc-by-2.0" by the UploadWizard Extension at the time it was transferred to Commons. See the license information for further details. |
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current | 15:11, 13 August 2014 | 2,480 × 1,780 (269 KB) | Meisam (talk | contribs) | User created page with UploadWizard |
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