File:Pbio.1000124.g002 (13951100900).png

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The OSD1 gene and protein.

(A)The OSD1 gene contains three exons and two introns and encodes a protein of 243 amino acids. The positions of the two Ds insertions are indicated by triangles. (B) A multiple sequence alignment of OSD1/UVI4 family members is shown, pointing out the segments of best conservation along OSD1. Particularly high conservation is observed in the central OSD1-motif, which is the only section with clear sequence similarity in moss and club-mosses. The conserved C-terminal 40–60 amino acids are predicted to contribute to alpha-helical structural elements. All available homologues are included for the selected range of species, with the species of origin being indicated by a two letter code preceding each protein identifier. Brassicaceae: Al, Arabidopsis lyrata; At, Arabidopsis thaliana; Br, Brassica rapa. Eurosids I: Gm, Glycine max; Pt, Populus trichocarpa. Poaceae: Os, Oryza sativa; Sb, Sorghum bicolor; Zm, Zea mays. Mosses: Pp, Physcomitrella patens. (C) Unrooted phylogenetic tree inferred from a OSD1/UVI4 family alignment using a neighbour-joining algorithm with pairwise gap removal. The full length sequences are included in the alignment except for Physcomitrella patens (Pp) sequences, for which only the conserved segment is aligned. The fact that only the aligning portions of Pp proteins were used in the reconstruction of the consensus tree topology should be taken into account during Pp-node branch length interpretation. Based on paralogue grouping, it can be hypothesised that gene duplication events occurred repeatedly in the evolution of plant OSD1/UVI4 family. The robustness of the topology is indicated by bootstrap confidence levels in percentage of 1,000 replicates. The analysis was performed with PHYLIPv3.66 (Felsenstein J (2006)) distributed by the author. Department of Genome Sciences, University of Washington, Seattle, United States of America) and PHYLO_WINv2.0 [32].
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Source pbio.1000124.g002
Author Phylogeny Figures

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This image was originally posted to Flickr by phylofigures at https://flickr.com/photos/123636286@N02/13951100900. It was reviewed on 19 December 2020 by FlickreviewR 2 and was confirmed to be licensed under the terms of the cc-by-2.0.

19 December 2020

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current13:23, 19 December 2020Thumbnail for version as of 13:23, 19 December 20202,264 × 2,916 (1.97 MB)Eyes Roger (talk | contribs)Transferred from Flickr via #flickr2commons

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