File:Panel for MicroScan WalkAway.jpg
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Captions
Captions
Panel for the MicroScan WalkAway microbial multitarget assay system
Summary
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| Description |
English: Panel for the MicroScan WalkAway microbial multitarget assay system, with machine in background. Bacteria isolated from a previously grown colony are distributed into each well, each of which contains growth medium as well as a the ingredients for a biochemical test, which will change the absorbance of the well depending on the bacterial property for the tested target. The panel will be incubated in a machine, which subsequently performs turbidimetry by sending light into each well, and interprets each one based on how much light was absorbed. The top wells are for Gram negative bacteria, and the bottom are for Gram positive bacteria. For interpretation, one of the two sides must be picked. The combined results will be automatically compared to a database of known results for various bacterial species, in order to generate a diagnosis of what bacterial species is present in the current panel. Simultaneously, it performs antibiotic susceptibility testing.
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| Date | |
| Source |
Ombelet S, Natale A, Ronat JB, Vandenberg O, Hardy L, Jacobs J (2021). "Evaluation of MicroScan Bacterial Identification Panels for Low-Resource Settings.". Diagnostics (Basel) 11 (2). DOI:10.3390/diagnostics11020349. PMID 33669829. PMC: 7922174. "This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/" |
| Author | Ombelet S, Natale A, Ronat JB, Vandenberg O, Hardy L, Jacobs J |
Licensing
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This file is licensed under the Creative Commons Attribution 4.0 International license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Wells
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| Abbre-viation | Substrate. | Panel |
|---|---|---|
| GLU | Glucose | Gram-negative |
| SUC | Sucrose | Gram-negative |
| INO | Inositol | Gram-negative |
| ADO | Adonitol | Gram-negative |
| RHA | Rhamnose | Gram-negative |
| MEL | Melibiose | Gram-negative |
| P4 | Penicillin G 4 µg/mL | Gram-negative |
| K4 | Kanamycin 4 µg/mL | Gram-negative |
| Cl4 | Colistin 4 µg/mL | Gram-negative |
| Cf8 | Cephalothin 8 µg/mL | Gram-negative |
| Fd64 | Nitrofurantoin 64 g/mL | Gram-negative |
| To4 | Tobramycin 4 g/mL | Gram-negative |
| CET | Cetrimide | Gram-negative |
| LYS | Lysine | Gram-negative |
| ORN | Ornithine | Gram-negative |
| TDA | Tryptophan Deaminase | Gram-negative |
| ESC | Esculin | Gram-negative |
| ONPG | o-Nitrophenyl-d-Galacto- pyranoside |
Gram-negative |
| CIT | Citrate | Gram-negative |
| MAL | Malonate | Gram-negative |
| ACE | Acetamide | Gram-negative |
| TAR | Tartrate | Gram-negative |
| OF/B | Oxidation Base Control | Gram-negative |
| OF/G | Oxidation of glucose | Gram-negative |
| H2S | Hydrogen Sulfide | Gram-negative |
| IND | Indole | Gram-negative |
| DCB | Decarboxylase Base Control | Gram-negative |
| LAC | Lactose | Gram-positive |
| TRE | Trehalose | Gram-positive |
| MNS | Mannose | Gram-positive |
| RBS | Ribose | Gram-positive |
| INU | Inulin | Gram-positive |
| MAN | Mannitol | Gram-positive |
| PGR | PNP-β-d-Glucuronide | Gram-positive |
| PGT | PNP-β-d-Galacto- pyranoside |
Gram-positive |
| IDX | Indoxyl Phosphatase | Gram-positive |
| PHO | Phosphatase | Gram-positive |
| PYR | Pyrrolidonyl-B-naphtylamide | Gram-positive |
| BE | Bile-Esculin | Gram-positive |
| PRV | Pyruvate | Gram-positive |
| BAC | Bacitracin | Gram-positive |
| CV | Crystal Violet | Gram-positive |
| MS | Bacitracin 0.05 g/mL | Gram-positive |
| NOV | Novobiocin 1.6 g/mL | Gram-positive |
| OPT | Optochin | Gram-positive |
| NACL | 6.5% NaCl | Gram-positive |
| SOR | Sorbitol | Both panels |
| RAF | Raffinose | Both panels |
| ARA | Arabinose | Both panels |
| ARG | Arginine | Both panels |
| NIT | Nitrate | Both panels |
| VP | Voges-Proskauer | Both panels |
| URE | Urea | Both panels |
File history
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 14:58, 9 February 2024 | | 1,391 × 1,077 (389 KB) | Mikael Häggström (talk | contribs) | Uploaded a work by Ombelet S, Natale A, Ronat JB, Vandenberg O, Hardy L, Jacobs J from {{cite journal| author=Ombelet S, Natale A, Ronat JB, Vandenberg O, Hardy L, Jacobs J| title=Evaluation of MicroScan Bacterial Identification Panels for Low-Resource Settings. | journal=Diagnostics (Basel) | year= 2021 | volume= 11 | issue= 2 | pages= | pmid=33669829 | doi=10.3390/diagnostics11020349 | pmc=7922174 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/... |
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