File:PGCs are closely associated with the complex fibronectin fibril meshwork in the germinal crescent. Quail.jpg
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| Description | Figure 5. PGCs are closely associated with the complex fibronectin fibril meshwork in the germinal crescent. Wholemount immunofluorescence for fibronectin (abB3/D6) and CVH across the germinal crescent of HH5 and HH6 [Tg(PGK1:H2B-mCherry)] quail embryos. (A) Fibronectin red, CVH (PGC marker, green), H2B-mCherry (blue) and merged maximum intensity projections of 10x tiled confocal Z stacks taken from the dorsal aspect of an HH6 embryo germinal crescent. Anterior (rostral) to top. HF = head fold. (A) Scale bar = 250 μm. Images in (B) are maximum intensity projections of 20x tiled confocal Z stacks taken from the dorsal aspect at the location of the bounding box in (A). (B) Scale bar = 50 μm. (C) Images are maximum intensity projections of 4 × 2.1 μm optical sections from 63x confocal Z stacks taken in an HH5 embryo. Yellow arrow in the CVH image highlights a bleb on a PGC. White arrow indicates a long cellular process. Scale bar = 25 μm. |
| Date | |
| Source | https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00035/full Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration. Front. Cell Dev. Biol. 7:35. doi: 10.3389/fcell.2019.00035 |
| Author | Huss DJ, Saias S, Hamamah S, Singh JM, Wang J, Dave M, Kim J, Eberwine J and Lansford R |
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© 2019 Huss, Saias, Hamamah, Singh, Wang, Dave, Kim, Eberwine and Lansford. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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| current | 10:08, 11 July 2024 | | 957 × 714 (854 KB) | Rasbak (talk | contribs) | {{Information |description=Figure 5. PGCs are closely associated with the complex fibronectin fibril meshwork in the germinal crescent. Wholemount immunofluorescence for fibronectin (abB3/D6) and CVH across the germinal crescent of HH5 and HH6 [Tg(PGK1:H2B-mCherry)] quail embryos. (A) Fibronectin red, CVH (PGC marker, green), H2B-mCherry (blue) and merged maximum intensity projections of 10x tiled confocal Z stacks taken from the dorsal aspect of an HH6 embryo germinal crescent. Anterior (ros... |
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