File:Origin-Dependent-Inverted-Repeat-Amplification-Tests-of-a-Model-for-Inverted-DNA-Amplification-pgen.webm
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[edit]DescriptionOrigin-Dependent-Inverted-Repeat-Amplification-Tests-of-a-Model-for-Inverted-DNA-Amplification-pgen.webm |
English: A two-dimensional animation of fork migration at short inverted repeats. The leading and lagging oligos (S1 Table) are drawn with the fold-back duplexes that create the nascent arms of the replication fork. The two oligos are held together by a 30 bp stretch of complementarity, reflecting the unreplicated parental duplex at a replication fork. The inverted repeats (in blue) lie in the single stranded Okazaki gap on the lagging strand. The animation steps through 25 steps of replication fork regression and illustrates the intermediates in which the 3’ C (in red) of the leading strand comes to lie next to the 5’T (in red) on the lagging, using the complementarity of the inverted repeats (in blue) as a template for annealing. There are 31 frames, numbered from 100 to 130, with branch migration beginning in frame 104. |
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Source | S1 Movie from Brewer B, Payen C, Di Rienzi S, Higgins M, Ong G, Dunham M, Raghuraman M (2015). "Origin-Dependent Inverted-Repeat Amplification: Tests of a Model for Inverted DNA Amplification". PLOS Genetics. DOI:10.1371/journal.pgen.1005699. PMID 26700858. PMC: 4689423. | ||
Author | Brewer B, Payen C, Di Rienzi S, Higgins M, Ong G, Dunham M, Raghuraman M | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:22, 28 January 2017 | 1 min 6 s, 480 × 640 (164 KB) | Yann (talk | contribs) | Imported media from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689423/bin/pgen.1005699.s002.mov |
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