File:Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s003.ogv
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Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s003.ogv (Ogg Theora video file, length 8.1 s, 150 × 146 pixels, 585 kbps, file size: 582 KB)
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[edit]DescriptionOptogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s003.ogv |
English: AktPH-mCh repeatedly moved to the plasma membrane upon photoactivation of the PI3K photoswitch. Time-lapse images of a HEK293 cell expressing AktPH-mCh and the PI3K photoswitch (plasmid #3). AktPH-mCh fluorescence images were acquired every 10 s. Photoactivation was given to whole cell with one pulse of a 442-nm HeCd laser (3%) at 0 min, 15 min and 30 min. Repeated activation successfully translocated AktPH-mCh to the plasma membrane. |
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Source | Movie S1 from Kakumoto T, Nakata T (2013). "Optogenetic Control of PIP3: PIP3 Is Sufficient to Induce the Actin-Based Active Part of Growth Cones and Is Regulated via Endocytosis". PLOS ONE. DOI:10.1371/journal.pone.0070861. PMC: 3737352. | ||
Author | Kakumoto T, Nakata T | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 05:59, 18 August 2013 | 8.1 s, 150 × 146 (582 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Kakumoto T, Nakata T |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | AktPH-mCh repeatedly moved to the plasma membrane upon photoactivation of the PI3K photoswitch. Time-lapse images of a HEK293 cell expressing AktPH-mCh and the PI3K photoswitch (plasmid #3). AktPH-mCh fluorescence images were acquired every 10 s. Photoactivation was given to whole cell with one pulse of a 442-nm HeCd laser (3%) at 0 min, 15 min and 30 min. Repeated activation successfully translocated AktPH-mCh to the plasma membrane. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |
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Categories:
- Cell motility
- Microfilaments
- Molecular cell biology
- Signal transduction
- Signaling cascades
- Polyphosphoinositide signaling cascade
- Signaling pathways
- Phosphoinositide signal transduction
- Cellular neuroscience
- Neuronal morphology
- Molecular neuroscience
- Synthetic biology
- Media from PLOS ONE
- Optogenetics
- Videos of laser-induced fluorescence