File:Optic cup development, gene expression, and schematic diagram of SFEBq culture.jpg
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| Description | Figure 1: Optic cup development, gene expression, and schematic diagram of SFEBq culture. (a) Schematic diagram of murine eye development from embryonic days E8—E11, a time in which the neural retina (NR) and retinal pigmented epithelial (RPE) tissues emerge. (b) Cryosections of an E10.5 mouse embryo underwent immunohistochemistry, selected gene expression displayed in red, DAPI shown in yellow, Actin shown in blue. Scale bar 100 μm. (c) Pseudocoloured E10.5 cryosection showing developing NR, RPE and lens. (d) Schematic diagram of SFEBq, a technique that generates ES-cell aggregates with a peripheral layer of Rx+ optic progenitor tissue. (e) Cryosections of Day 10 SFEBq Rx:GFP aggregate with a continuous peripheral layer of optic progenitor tissue underwent immunohistochemistry. Marker gene expression displayed in red, DAPI shown in yellow, Actin shown in blue. Scale bar 100 μm. (f) Pseudocoloured diagram of a Day 10 SFEBq aggregate with differentiating NR-like and RPE-like tissues. |
| Date | |
| Source | https://www.nature.com/articles/sdata201530 Andrabi, M., Kuraku, S., Takata, N. et al. Comparative, transcriptome analysis of self-organizing optic tissues. Sci Data 2, 150030 (2015). https://doi.org/10.1038/sdata.2015.30 |
| Author | Andrabi, M., Kuraku, S., Takata, N. et al. |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 17:58, 15 September 2024 | | 1,615 × 1,928 (1.86 MB) | Rasbak (talk | contribs) | {{Information |description=Figure 1: Optic cup development, gene expression, and schematic diagram of SFEBq culture. (a) Schematic diagram of murine eye development from embryonic days E8—E11, a time in which the neural retina (NR) and retinal pigmented epithelial (RPE) tissues emerge. (b) Cryosections of an E10.5 mouse embryo underwent immunohistochemistry, selected gene expression displayed in red, DAPI shown in yellow, Actin shown in blue. Scale bar 100 μm. (c) Pseudocoloured E10.5 cryosec... |
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