File:OSC Microbio 09 02 listumbrel.jpg

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Name: Microbiology ID: e42bd376-624b-4c0f-972f-e0c57998e765@4.4 Language: English Summary: Subjects: Science and Technology Keywords: Print Style: License: Creative Commons Attribution License (by 4.0) Authors: OpenStax Microbiology Copyright Holders: OpenStax Microbiology Publishers: OpenStax Microbiology Latest Version: 4.4 First Publication Date: Oct 17, 2016 Latest Revision: Nov 11, 2016 Last Edited By: OpenStax Microbiology 1977 Richard R. Facklam, Ph.D.

This 1977 photograph depicted two Petri dishes each filled with trypticase soy agar medium containing 5% defibrinated sheep's blood, i.e., blood agar plate (BAP). The plate on the left had been stabbed and streaked with an inoculum containing Streptococcus mitis, alpha-hemolytic bacteria, a member of the Viridans group, while the right plate was stabbed with an inoculum containing Group A Streptococcus pyogenes (GAS), a typical beta-hemolytic bacteria. The inoculation was performed using a wire loop, which had been dipped into a primary culture medium. The BAPs were incubated in a carbon dioxide enriched atmosphere at 35oC for 24 hours. There is no magnification of this image.

All culture organisms were Gram-positive cocci bacteria. The alpha-hemolytic organisms in the left plate grew colonies that were surrounded by a "hazy", indistinct zone of partial red blood cell (RBC) destruction, i.e., hemolysis, and the beta-hemolytic bacteria on the right grew colonies surrounded by a clear, colorless zone of complete RBC destruction. Using the "stabbing" method of inoculation makes the qualitative interpretation of the hemolytic reaction much easier, for the results of colonial interactivity with the blood agar medium is much more pronounced, and therefore, easier to discern.

Infection with GAS can result in a range of symptoms:

- No illness

- Mild illness (strep throat or a skin infection such as impetigo)

- Severe illness (necrotizing faciitis, streptococcal toxic shock syndrome)
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Source https://cnx.org/contents/5CvTdmJL@4.4
Author CNX OpenStax

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