File:Neuroshperes with ZIKV infection.jpg
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![File:Neuroshperes with ZIKV infection.jpg](https://upload.wikimedia.org/wikipedia/commons/thumb/7/7f/Neuroshperes_with_ZIKV_infection.jpg/753px-Neuroshperes_with_ZIKV_infection.jpg?20220406205702)
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Graphs and images depicting ZIKV infection in twins
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[edit]DescriptionNeuroshperes with ZIKV infection.jpg |
English: Graphs and images depicting ZIKV infection in twins
Description on original article (CC-BY 4.0): https://www.nature.com/articles/s41467-017-02790-9 NPC characterization and ZIKVBR infection in DZ-D cells (see also Supplementary Fig. 1). a, b NPCs were stained with isotype IgG APC-A and isotype IgG FITC-A (a) and Nestin APC-A and Musashi-1 FITC-A (b) and were analyzed by flow cytometry to confirm (or show) population homogeneity for neural progenitor markers. c Nestin staining by immunofluorescence. Scale bar (SB), 20 μm d Representative phase-contrast micrograph of infected NPCs (#10608 twins). SB, 100 μm. e Analysis of cell number of Mock-infected NPCs from non-affected and CZS-affected twins (n = 3 technical replicates; mean ± SEM). f, g MOI 0.01 and 0.1 infected NPCs from non-affected (#10608-4, #10763-4, and #10788-4) (f) and CZS-affected (#10608-1, #10763-1, and #10788-1) (g) twins in monolayer cultures at 24, 48, 72, and 96 hpi (n = 3 technical replicates; mean ± SEM; *p < 0.05, **p < 0.01; two-way ANOVA with Bonferroni post hoc analysis). h Representative images of neurospheres infected with ZIKVBR (#10608 twins, n = 2 technical replicates). SB, 400 μm. i, j Normalized diameter of neurospheres at 24 and 96 hpi with MOI 0.01 (i) or MOI 0.1 (j); #10608, #10763 and #10788 twins; n ≅ 15 technical replicates; mean ± SEM; *p < 0.05, **p < 0.01 Student’s t test |
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Source | https://www.nature.com/articles/s41467-017-02790-9 |
Author | Luiz Carlos de Caires Junior, Ernesto Goulart, Uirá Souto Melo, Bruno Henrique Silva Araujo et al. |
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current | 20:57, 6 April 2022 | ![]() | 806 × 642 (178 KB) | TiagoLubiana (talk | contribs) | Cropped 59 % horizontally, 75 % vertically using CropTool with precise mode. |
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