File:Neopestalotiopsis species.jpg

English: General Information

MycoBank Typification #(MBT) 10003037 Status Holotype Identified as Neopestalotiopsis psidii I.U. Haq, S. Ijaz & N.A. Khan, Pakistan Journal of Agricultural Sciences 58 (4): 1306 (2021) [MB#840638] Strain numbers FMBCC 11.2= FMB0028 Collected by I.U. Haq Collection date 11/04/2017 Location details Punjab Locality (country, state, city, etc) Pakistan Host Psidium guajava Substrate details Living branches of Guava tree

Description Neopestalotiopsis psidii The Colonies on the PDA medium after seven days at 27- 28°C appeared with white to grey moderate aerial mycelium, lobate edge, black colored gregarious conidiomata on mycelium's surface. Conidia were straight to slightly curved, fusoid with four septations having 24±1.0 µm length and 6.6±0.7 µm width, having four septations with three versicolourous median cells. The first two median cells were honey yellow colored having length of 5±0.5 µm and 5.3±0.6 µm, respectively, while the third median cell was light yellow and was 4.8±0.6 µm long. Septa were darker than the rest of the cells. The apical cell was hyaline and cylindrical, having a length of 4.3±0.6 µm. Two tubular apical appendages of length 20.5±5.6 µm length were raised from the top of the apical cell. The basal cell was obconic to conical, having 4.4±0.4 µm length. Straight to curved basal appendage was produced with 4±1.5 µm length.

General Information: MycoBank Typification #(MBT) 10003040 Status Holotype Identified as Neopestalotiopsis guajavae I.U. Haq, S. Ijaz & N.A. Khan, Pakistan Journal of Agricultural Sciences 58 (4): 1306 (2021) [MB#840596] Strain numbers FMBCC 11.1= FMB0026-FMB0027 Herbarium records FMB H 11.1 holotype Collected by I.U. Haq Collection date 11/04/2017 Location details Punjab Locality (country, state, sity, etc) Pakistan Host Psidium guajava Substrate details Living branches of Guava tree

Description Neopestalotiopsis guajavae Colonies after seven days of inoculation at 27-28, °C with white-colored concentric aerial mycelium and black conidiomata with undulating edge appeared on the PDA medium. The conidia were straight to slightly curved with four septations, discrete cells having 23.1±1.4 µm length and 7.2 ± 0.4 µm width. Conidia produced versicolourous three median cells. The first two median cells were brown, having 4.8 ± 0.4 µm and 5.3±0.4 µm length respectively, while the third cell was light yellow with 5.1±0.3 µm length. Septa were darker in colour than the rest of the cells. The apical cell was V-shaped, hyaline smooth and thin-walled, having a length of 3.9±0.7 µm. One to two tubular apical appendages 20.8±4.1 µm long were raised from the top of the apical cell. The conical basal cell was 3.7±0.6 µm long with a thin rugose wall. The basal appendage was filiform, straight to slightly curved, 5.7 ± 1.2 µm long

General Information: MycoBank Typification #(MBT) 10003039 Status Holotype Identified as Neopestalotiopsis guajavicola I.U. Haq, Ijaz & N.A. Khan, Pakistan Journal of Agricultural Sciences 58 (4): 1307 (2021) [MB#840639] Strain numbers FMBCC 11.4= FMB0129 Herbarium records FMB H 11.4 holotype Collected by I.U. Haq Collection date 11/04/2017 Location details Punjab Locality (country, state, sity, etc) Pakistan Host Psidium guajava Substrate details Living branches of Guava tree

Description Neopestalotiopsis guajavicola Colonies of isolate FMB-Guv-L2A produced undulating edge white to honey-coloured moderate aerial mycelium with black-colored gregarious conidiometa on the PDA medium after seven days at 27-28°C. The Conidia were straight, relatively broader, ellipsoid, clavate having length 23.3±1.6 µm and width 6.5±0.5 µm. The conidia were containing four Septations and three median cells. The first two median cells were yellow to brown, having 4.8±0.3 µm and 5.1±0.2 µm length respectively, the third cell was light yellow with 4.8±0.5 µm length. Septa were darker than the rest of the cell. The apical cell was relatively small, hyaline having 4.4±0.4 µm length with a very thin wall. Two to three tubular unbranched apical appendages having 21.8±2.7 µm length were produced. In addition, a conical basal cell 4.0±0.5 µm long with a single unbranched basal appendage 4.4±1.5 µm in length was observed.

General Information: MycoBank Typification #(MBT) 10003038 Status Holotype Identified as Neopestalotiopsis perukae I.U. Haq, Ijaz & N.A. Khan, Pakistan Journal of Agricultural Sciences 58 (4): 1306 (2021) [MB#840637] Strain numbers FMBCC 11.3= FMB0127- FMB0128- FMB0130 Herbarium records FMB H 11.3 holotype Collected by I.U. Haq Collection date 11/04/2017 Location details Punjab Locality (country, state, sity, etc) Pakistan Host Psidium guajava Substrate details Living branches of Guava tree

Description Neopestalotiopsis perukae

Colonies on the PDA medium after seven days at 27-28°C were whitish to pale white dense aerial mycelium with the smooth edge with yellowish gregarious conidiomata immersed in the mycelium. Conidia were straight to slightly curved, fusiform, ellipsoid having length 19.7±1.4 µm and width 6.4±0.8 µm. Conidia were produced with four septations and three versicolourous median cells. The upper two median cells were dark brown to black, with 4.35±0.4 µm and 4.4±0.4 µm length respectively, the third median cell was pale yellow and 4.6±0.6 µm long. The wall between the upper two median cells was relatively thicker and darker, sub cylindrical hyaline thin-walled apical cell with 3.6 ± 0.7 µm length. Two unbranched, un-knobbed tubular apical appendages attached to the middle portion of the apical cell were 22.2±5.8 µm long. The hyaline obconic basal cell was 3.1 ± 0.5 µm long, with a posterior appendage of 3.8 ± 1.9 µm length.