File:Morphology and anatomical properties of Glutamatergic Ventro-Lateral Interneurons.png

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Fig 2. Morphology and anatomical properties of GVLIs.

(A-F) Morphology of GVLIs as visualized with mCD8::GFP expressed under the control of R26F05-Gal4 (A-C) or R26A08-Gal4 (D-F). (A, D) Stacked images of the whole VNC, dorsal view. (B, E) Enlarged views of dorsal region of the neuropile in A3 to A5, co-stained for Fas2 (blue) and vGluT (magenta). Arrowheads indicate TP1 projections. vGluT localization was observed in the neurites of GVLIs (arrows). (C, F) Localization of VGluT in neurites of GVLIs in a single optical section of 1.11 μm thickness. (G-J) Localization of Syt::GFP in GVLIs. Expression driven by R26F05-Gal4 (G, H) or R26A08-Gal4 (I, J). (G, I) Stacked images of the VNC. Syt::GFP staining is largely confined to the dorso-medial neurites of GVLIs. (H, J) Colocalization of Syt::GFP with vGluT. (K-N) Mosaic labeling of GVLIs with myr-GFP (K-M) and a diagram of a single GVLI (N). (L) Putative axon terminals are seen at both ipsi- and contralateral side (enclosed by dotted lines in K). Yellow arrows and white arrows indicate putative dendritic sites. One of them in a medial position (yellow arrows) extended to the anterior neuromere (yellow arrows, beyond TP1 projections (arrowheads)). The cell body is indicated by asterisk. Scale bars, 20 μm. https://doi.org/10.1371/journal.pone.0136660.g002
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Source https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0136660 Itakura Y, Kohsaka H, Ohyama T, Zlatic M, Pulver SR, Nose A (2015) Identification of Inhibitory Premotor Interneurons Activated at a Late Phase in a Motor Cycle during Drosophila Larval Locomotion. PLoS ONE 10(9): e0136660. https://doi.org/10.1371/journal.pone.0136660
Author Itakura Y, Kohsaka H, Ohyama T, Zlatic M, Pulver SR, Nose A
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current00:51, 9 September 2024Thumbnail for version as of 00:51, 9 September 20241,702 × 2,308 (3.51 MB)Rasbak (talk | contribs){{Information |description=Fig 2. Morphology and anatomical properties of GVLIs.<br> (A-F) Morphology of GVLIs as visualized with mCD8::GFP expressed under the control of R26F05-Gal4 (A-C) or R26A08-Gal4 (D-F). (A, D) Stacked images of the whole VNC, dorsal view. (B, E) Enlarged views of dorsal region of the neuropile in A3 to A5, co-stained for Fas2 (blue) and vGluT (magenta). Arrowheads indicate TP1 projections. vGluT localization was observed in the neurites of GVLIs (arrows). (C, F) Local...

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