File:LINE reverse transcription.png
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[edit]DescriptionLINE reverse transcription.png |
English: Mechanism of target-primed reverse transcription (TPRT), directly at the site of integration. L1 RNP recognize AAAATT hexanucleotides and ORF2 endonuclease activity cleaves the DNA first-strand. L1 polyA tail associate with TTTT overhang and the host DNA is used as a primer to initiate reverse-transcription. ORF2 probably also mediate second-strand cleavage and attachment of newly synthesized cDNA to the DNA template, using again host DNA as a primer for second-strand synthesis. |
Date | |
Source | Own work |
Author | Mariuswalter |
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current | 01:58, 28 September 2017 | 3,713 × 5,101 (416 KB) | Mariuswalter (talk | contribs) | User created page with UploadWizard |
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