File:Jak2 knockdown affects buccopharyngeal membrane and cranial muscles of Xenopus laevis.png
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| Description | Figure 3. Jak2 knockdown affects buccopharyngeal membrane and cranial muscles. A-E) Frontal view of the face of representative embryos at stage 40-41 showing a ruptured or persisting buccopharyngeal membranes (images from n=60, 3 biological replicates for each treatment). In the bottom right corner shows an image of the injection site/stage. Prime labeled images show magnified images of the mouth and the double prime labeled images show the same images with the buccopharyngeal membrane shaded blue. F) Shows relative percentages of embryos with an intact, partially intact or absent buccopharyngeal membrane (n=60 for each group, 3 biological replicates). G-H) Optical sagittal section through the head of representative controls and jak2 morphants. The buccopharyngeal membrane is absent in control and is present but thin in the jak2 morphants (n=12, 2 biological replicates). I-L) Representative thick agarose section through the face showing phosphor-`JAK2 and 12/101 immunofluorescence. I) phospho-Jak2 (red), J) 12/101=muscle specific antibody (green), K) DAPI (blue), L) merge. M,N) Lateral views of representative embryos showing 12/101 muscle labeling (red) in control and jak2 morphants and counterstained with DAPI (blue). White arrows point to the location. Xenopus laevis. nos=nostril, MO=morpholino, BPM=buccopharyngeal membrane. |
| Date | |
| Source | https://www.researchgate.net/publication/369979586_Jak2_and_Jaw_Muscles_Are_Required_for_Mouth_Development Dickinson, A.J.G. Jak2 and Jaw Muscles Are Required for Buccopharyngeal Membrane Perforation during Mouth Development. J. Dev. Biol. 2023, 11, 24. |
| Author | Amanda J.G. Dickinson |
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| current | 10:19, 26 June 2024 | | 750 × 1,007 (818 KB) | Rasbak (talk | contribs) | {{Information |description=Figure 3. Jak2 knockdown affects buccopharyngeal membrane and cranial muscles. A-E'') Frontal view of the face of representative embryos at stage 40-41 showing a ruptured or persisting buccopharyngeal membranes (images from n=60, 3 biological replicates for each treatment). In the bottom right corner shows an image of the injection site/stage. Prime labeled images show magnified images of the mouth and the double prime labeled images show the same images with the bu... |
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