File:In-situ hybridisation on chicken embryo.jpg
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Afrikaans: In hierdie prent kan ons 'n hoenderembrio sien wat gebruik is vir 'n in situ hibridisasie-eksperiment. Wat ons sien is dat die selle waar MyoD manifesteer, gekleurd is. Die gekleurde selle is dus dié wat sal deelneem aan die ontwikkeling van die skeletspiere. Die beeld is met my iPhone deur die mikroskooplens vasgevang.
In-situ hibridisasie stel 'n eksperiment in staat om die uitdrukking van 'n spesifieke geen in die weefsel/embrio van belang te lokaliseer. Die sterkpunt van hierdie tegniek is dat dit resolusie tot op sellulêre vlak kan weergee sonder die behoefte aan gespesialiseerde toerusting en reagense. In hierdie eksperiment het ons gesoek na die MyoD mRNA, om te sien waar en wanneer die geen manifesteer. Ons het hierdie eksperiment gemaak om spierontwikkeling en groei te bestudeer. Ons aandag was gefokus op hipertrofiese diere en hul gebruik in soötegnologie, en ons het aldus spiere en die gene wat met hul groei verband hou bestudeer. Ek is 'n student in agronomiese en omgewingsbiotegnologie aan die Universiteit van Perugia in Italië. English: In this picture we can see a chicken embryo that is been used for an in situ hybridization experiment. What we see is that the cells were MyoD is expressed are coloured. So the coloured cells are the one that will participate in the development of the skeletal muscles. The image was captured with my iPhone trough the microscope lens. In-situ hybridisation allows an experimenter to localize the expression of a particular gene in the tissue/embryo of interest. The strength of this technique is that it can give resolution down to a cellular level without the need for specialised equipment and reagents. In this experiment we were looking for the MyoD mRNA, to see where and when the gene was expressed. We made this experiment to study muscle development and growth. Our attention was focused on hypertrophic animals and their use in zootechnics, so we were studying muscles and the genes related to their growth. I'm a student in Agronomic and Environmental Biotechnology at the Università degli Studi di Perugia (University of Perugia), in Italy. |
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| Source | Own work |
| Author | Simone Castellana |
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I, the copyright holder of this work, hereby publish it under the following license:
This file is licensed under the Creative Commons Attribution-Share Alike 4.0 International license.
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This image was uploaded as part of European Science Photo Competition 2015. |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 18:37, 3 November 2015 | | 3,264 × 2,448 (1.87 MB) | Koukoo (talk | contribs) | User created page with UploadWizard |
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