File:IRM Interference Reflection Microscopy.svg

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Summary

Description	English: Principle of Interference Reflection Microscopy (IRM) showing interference effect on reflected wave fronts and the result on the final image intensity. Bright green wave represents the polarized light from the light source. The darker green waves are the reflections from the cell membrane, the blue waves the reflections from the glass surface. If the membrane is close to the glass, the reflected waves are shifted about half a wavelength (see red line) and will cause interference, resulting in a dark pixel. If there is more distance between the membrane and the glass, the returning waves will be less shifted and won't cause interference, resulting in a brighter pixel in the final image. Indicated are the typical refractive indices of the glass, medium and the cell membrane, which determine the amount of reflection.
Date	14 February 2010
Source	Own work
Author	Jhpbroeke
Other versions	File:Interference_Reflection_Microscopy.svg

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File history

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	Date/Time	Dimensions	User	Comment
current	13:34, 24 March 2010	524 × 269 (366 KB)	Jhpbroeke (talk \| contribs)	Reverted to version as of 13:31, 24 March 2010
	13:31, 24 March 2010	524 × 269 (366 KB)	Jhpbroeke (talk \| contribs)	Reverted to version as of 13:26, 24 March 2010
	13:31, 24 March 2010	524 × 269 (366 KB)	Jhpbroeke (talk \| contribs)	Reverted to version as of 13:28, 24 March 2010
	13:31, 24 March 2010	744 × 1,052 (366 KB)	Jhpbroeke (talk \| contribs)	Reverted to version as of 13:22, 24 March 2010
	13:28, 24 March 2010	524 × 269 (366 KB)	Jhpbroeke (talk \| contribs)	Reverted to version as of 13:24, 24 March 2010
	13:26, 24 March 2010	524 × 269 (366 KB)	Jhpbroeke (talk \| contribs)	colors substituted
	13:24, 24 March 2010	524 × 269 (366 KB)	Jhpbroeke (talk \| contribs)	decreased the document area: less blank space
	13:22, 24 March 2010	744 × 1,052 (366 KB)	Jhpbroeke (talk \| contribs)	phase shift added and colors adjusted
	00:13, 22 February 2010	524 × 269 (390 KB)	Jhpbroeke (talk \| contribs)	Zoomed in version, corrected the interference, included the refractive indices of the principal components and added a cell membrane. ~~~~
	15:49, 14 February 2010	654 × 304 (36 KB)	Jhpbroeke (talk \| contribs)	{{Information \|Description={{en\|1=Principle of Interference Reflection Microscopy (IRM) showing interference effect on reflected wave fronts and the result on the final image intensity. Bright green wave represents the polarized light from the light sourc