File:Histological staining following imaging of lung tissue of BM05.webp
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![File:Histological staining following imaging of lung tissue of BM05.webp](https://upload.wikimedia.org/wikipedia/commons/thumb/6/60/Histological_staining_following_imaging_of_lung_tissue_of_BM05.webp/800px-Histological_staining_following_imaging_of_lung_tissue_of_BM05.webp.png?20211213215813)
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From the study "Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography"
Summary
[edit]DescriptionHistological staining following imaging of lung tissue of BM05.webp |
English: "H&E and IHC staining of COVID-19 lung biopsy and control (Donor 4) lung biopsy after HiP-CT imaging. H&E staining of overview, parenchyma and alveoli in columns 1-3; CD31 (column 4), Thyroid transcription factor 1 (TTF-1 (column 5)) and Fibrin staining (column 6) (N=1). For histological comparison, representative lung samples were cut and embedded in paraffin. 2 µm thick sections were cut followed by histological staining using Hematoxylin and Eosin (HE) at the Institute of Pathology at Hannover Medical School. Immunohistochemistry for CD31 1:75 (M0823, Agilent Dako, California, USA), TTF-1 RTU (790-4756, Hoffmann-La Roche, Basel, Swiss) and Fibrin 1:500 (MABS2155, Merck Millipore, Massachusetts, USA) were stained on a VENTANA BenchMark ULTRA (Hoffmann-La Roche, Basel, Swiss) with the aforementioned dilutions and pretreatment and incubation times according to the manufacturers advice. Representative images were acquired with a Olympus CS50 camera (Olympus, Tokyo, Japan) using Olympus cellSens Software (Olympus, Tokyo, Japan) on a routine diagnostic light microscope (BX43, Olympus, Tokyo, Japan)." |
Date | |
Source | https://www.nature.com/articles/s41592-021-01317-x |
Author | Authors of the study: C. L. Walsh, P. Tafforeau, W. L. Wagner, D. J. Jafree, A. Bellier, C. Werlein, M. P. Kühnel, E. Boller, S. Walker-Samuel, J. L. Robertus, D. A. Long, J. Jacob, S. Marussi, E. Brown, N. Holroyd, D. D. Jonigk, M. Ackermann & P. D. Lee |
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current | 21:58, 13 December 2021 | ![]() | 2,126 × 738 (322 KB) | Prototyperspective (talk | contribs) | Uploaded a work by Authors of the study: C. L. Walsh, P. Tafforeau, W. L. Wagner, D. J. Jafree, A. Bellier, C. Werlein, M. P. Kühnel, E. Boller, S. Walker-Samuel, J. L. Robertus, D. A. Long, J. Jacob, S. Marussi, E. Brown, N. Holroyd, D. D. Jonigk, M. Ackermann & P. D. Lee from https://www.nature.com/articles/s41592-021-01317-x with UploadWizard |
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