File:Histological staining following imaging of lung tissue of BM05.webp

English: "H&E and IHC staining of COVID-19 lung biopsy and control (Donor 4) lung biopsy after HiP-CT imaging. H&E staining of overview, parenchyma and alveoli in columns 1-3; CD31 (column 4), Thyroid transcription factor 1 (TTF-1 (column 5)) and Fibrin staining (column 6) (N=1). For histological comparison, representative lung samples were cut and embedded in paraffin. 2 µm thick sections were cut followed by histological staining using Hematoxylin and Eosin (HE) at the Institute of Pathology at Hannover Medical School. Immunohistochemistry for CD31 1:75 (M0823, Agilent Dako, California, USA), TTF-1 RTU (790-4756, Hoffmann-La Roche, Basel, Swiss) and Fibrin 1:500 (MABS2155, Merck Millipore, Massachusetts, USA) were stained on a VENTANA BenchMark ULTRA (Hoffmann-La Roche, Basel, Swiss) with the aforementioned dilutions and pretreatment and incubation times according to the manufacturers advice. Representative images were acquired with a Olympus CS50 camera (Olympus, Tokyo, Japan) using Olympus cellSens Software (Olympus, Tokyo, Japan) on a routine diagnostic light microscope (BX43, Olympus, Tokyo, Japan)."