File:Hand-book of physiology (1892) (14762024621).jpg

Identifier: handbookofphysio00bake (find matches)
Title: Hand-book of physiology
Year: 1892 (1890s)
Authors: Baker, W. Morrant, (William Morrant), 1839-1896 Harris, Vincent Dormer Kirkes, William Senhouse, 1823-1864. Hand-book of physiology. 13th ed
Subjects: Physiology Human physiology
Publisher: London : John Murray
Contributing Library: Francis A. Countway Library of Medicine
Digitizing Sponsor: Open Knowledge Commons and Harvard Medical School

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ge of the instrument. Thecomparison wedge, K, is so placed that one of its longitudinaledges bisects the circular stage-opening, so that one-half of thelatter is cut off by the red-tinted wedge. Into the stage-openingfits a small circular trough, G, having a glass bottom, and dividedinto equal compartments by a thin lamina. One compartment, a,is filled in the manner to be presently indicated with diluted CH. V.) AMOUNT OF HEMOGLOBIN. 159 blood, and the other, a, with water, the trough is so placed thatthe lamina is in one plane with the edge of the wedge, the watercompartment being above the wedge and the blood compartmentabove the free half of the stage opening. By turning the screwhead, T, the frame, P, with the wedge, K, may be moved back-wards and forwards until a position is found where the intensityof the tints due to the stratum of blood on the one hand and thethickness of the wedge on the other appears to be equal. Therequired degree of dilution is obtained by the use of small
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Fig. 1: -FleiscKLs Hsemoglobinometer. capillary tubes of a capacity varying from 6 to 8 cmm. Thecapillary pipette is filled with blood and is held over the bloodcompartment and its contents thoroughly washed out into thatcompartment, and the blood and water are mixed with a wire.Water is then added until the blood compartment is quite full.The other compartment is filled with water. Light is thenreflected by the mirror, S, so as to illuminate both compartments.By moving K by means of the milled head, T, sl position of Kmay be found corresponding to the exact intensity of the lightpassing through the two compartments; this is read off at M onthe scale P, the division of which corresponds to standard strengthsof solutions of haemoglobin. Distribution of Haemoglobin.—Haemoglobin occurs not onlyin the red blood-cells of all Vertebrata (except amphioxus andleptocephalus whose blood-cells are all colourless), but also in l60 THE BLOOD. (ch. v. similar cells in many Worms ; moreover, it is

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