File:GE23077-binds-to-the-RNA-polymerase-i-and-i+1-sites-and-prevents-the-binding-of-initiating-elife02450f003.jpg

English: (A) Overall structure. Green, GE; violet sphere, Mg2+(I); yellow, σ. (B) Crystallographic data and refinement statistics. (C) Electron density and atomic model for GE. Blue mesh, mFo-DFc omit map for GE (contoured at 2.5σ); blue sticks, GE; gray ribbons, RNAP backbone; green surfaces, RNAP residues at which substitutions confer GE-resistance (Figure 2A; Supplementary file 2C); cyan sticks, additional RNAP residues that contact GE; gray and red sticks additional RNAP residues that coordinate Mg2+(I); violet sphere, Mg2+(I). D2, LR, H, I, Mg2+, and BH denote the RNAP D2 loop, link region, H region, I region, Mg2+ loop, and bridge helix. RNAP residues are numbered both as in T. thermophilus RNAP and as in E. coli RNAP (in parentheses). (D) Contacts between RNAP and GE (stereodiagram). Gray ribbons, RNAP backbone; gray sticks, RNAP carbon atoms; green, GE carbon atoms; red, oxygen atoms; blue, nitrogen atoms; red spheres, water molecules; violet sphere, Mg2+(I). Blue dashed lines, H-bonds; orange dashed lines, coordinate-covalent bonds. (E) Contacts between RNAP and GE (schematic). Red dashed lines, H-bonds; orange dashed lines, coordinate-covalent bonds; blue arcs, van der Waals interactions; W, water molecule. See Figure 3—figure supplement 1.