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English: Molecular phylogenetic analysis of the MEX1 proteins. The evolutionary history was inferred by using the maximum likelihood method based on the WAG model (Whelan and Goldman, 2001). Sequence alignment was performed with MUSCLE included in MEGA5 (Tamura et al., 2011). Alignment quality was assessed by GUIDANCE giving an alignment score of 0.783. The bootstrap consensus tree inferred from 500 replicates is taken to represent the evolutionary history of the taxa analyzed. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown next to the branches. A discrete gamma distribution was used to model evolutionary rate differences among sites (five categories, +G, parameter = 3.3094). The rate variation model allowed for some sites to be evolutionarily invariable ([+I], 5.4532% sites). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 14 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 173 positions in the final dataset. Evolutionary analyses were conducted in MEGA5 (Tamura et al., 2011).