File:Fpls-02-00047-g002.png

English: Effect of ORA59 on the differential JA response during P. rapae feeding. (A) RT-qPCR analysis of ORA59 transcript levels (relative to At1g13320) in non-treated and P. rapae-infested Arabidopsis Col-0 and jin1-2 plants at 24, 30, 36, 48, and 72 h after removal of P. rapae caterpillars that had fed on the tissue for 24 h. Transcript levels relative to non-treated Col-0 at t = 24 h, which was set at 1, are plotted. (B) Northern blot analysis of PDF1.2, VSP2, ORA59, and MYC2 transcript levels in Col-0, RNAi-ORA59, jin1-1 and jin1-1/RNAi-ORA59 plants at different time points after P. rapae feeding. (C) Northern blot analysis of PDF1.2 and VSP2 transcript levels in Col-0, jin1-7 and 35S:ORA59 plants at different time points after P. rapae feeding. To check for equal loading, rRNA bands were stained with ethidium bromide. The experiments were repeated with similar results.