File:Fpls-02-00042-g008.png

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English: Immunoblot of XylT_RNAi tomato fruits using IgE from Lyc e 2-sensitized patients. IgE-binding to soluble protein extracts from red tomato fruits harvested from L. esculentum cv. MicroTom wild type (WT, lanes 1 and 6), XylT_RNAi line 3 (lanes 2–5), XylT_RNAi 12 (lanes 7–10). (A) IgE-binding using serum from patient 7241–7, (B) IgE-binding using serum from patient 8695–24. Fruits analyzed were derived from the T1-generation of transgenic plants. Lane 2, XylT_RNAi 3–1; lane 3, XylT_RNAi 3–7; lane 4, XylT_RNAi 3–10; lane 5, XylT_RNAi 3–16; lane 7, WT; lane 8, XylT_RNAi 12–3; lane 9, XylT_RNAi 12–4; lane 10, XylT_RNAi 12–12. (C) SDS-PAGE and silver staining of protein extracts from red fruits. Arrow heads indicate protein standards of 95, 55, and 36 kDa, respectively.
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Source doi:10.3389/fpls.2011.00042
Author Kathrin Elisabeth Paulus, Vera Mahler, Martin Pabst, Karl-Heinz Kogel, Friedrich Altmann, Uwe Sonnewald

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