File:Fpls-02-00020-g001.png

English: Matrix illustrating the Arabidopsis ESCRT interactome based on the yeast two-hybrid assay. Plasmids encoding selected GAL4-AD and -BD Arabidopsis ESCRT fusion proteins (as well as the corresponding “empty” AD and BD plasmids) were co-transformed into yeast, then replica plated on either low- or high-stringency selection media with or without 3-AT. Those BD-ESCRT fusion constructs that were not further tested in yeast two-hybrid (growth) assays because they displayed growth when co-expressed with the “empty” AD plasmid (i.e., autoactivation) on selective media with or without 3-AT, are indicated with solid squares or circles, respectively. Shaded boxes represent the relative rates of yeast growth on high-stringency selection media (with 3-AT) as either: strong (black), medium (dark gray), weak (light gray), or no growth (white). Also indicated in the matrix are the averages of the measured β-Gal activities for each positive-interacting protein pair, with the exception of those interactions in which yeast strains that harbored certain BD-ESCRT plasmids along with the “empty” AD plasmid displayed growth (autoactivation) on low-selection media alone (indicated as “nd”). Note that the relative growth rates of most yeast strains expressing a positive-interacting protein pair are generally proportional to their calculated β-Gal activities.