File:Fpls-02-00013-a002.png

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English: Vicia faba L., minor cv. Scirocco (Saaten-Union GmbH, Isernhagen, Germany). Calibration curve for Oregon Green 488 dextran fluorescence excitation ratio R (495ex/440ex; 525em). The Boltzmann fit was chosen for fitting sigmoidal curves to in situ calibration ratio data. Fitting resulted in an optimal dynamic range for pH measurements between 3.9 and 6.3 (corresponds to the ratios 1.1 and 3.6). In situ calibration was conducted on six different plants (n = 6 biological replicates), each biological replicate was technically replicated a further five times (n = 5 technical replicates), whereas at least five regions of interest were averaged to yield one technical replicate (n ≥ 5 ROI). Data are mean of the six biological replicates ± SE.
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Source doi:10.3389/fpls.2011.00013
Author Christoph-Martin Geilfus, Karl H. Mühling

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