File:Fcimb-13-1195803-g001.jpg

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Fluorescence in situ hybridization

Summary

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Description
English: Figure 1 Steps of traditional FISH protocol (I). First, the biofilm sample is fixed to stabilize the cells and permeabilize the cell membrane (1). Then, labelled probes are added and allowed to hybridize with the rRNA target (2), and the excess probe is washed away (3). Finally, the sample is analyzed under epifluorescence microcopy or confocal laser scanning microscopy to determine the spatial distribution of biofilm populations (4). Species in polymicrobial biofilms can be organize in three different ways (II): (A) separate microcolonies (confocal microscopy image reprinted with permission from (Mark Welch et al., 2016)); (B) co-aggregation (confocal microscopy image reprinted with permission from (Azevedo et al., 2016)); (C) arranged in layers (confocal microscopy image reprinted with permission from (Almeida et al., 2011)). Created with BioRender.com.
Date
Source https://www.frontiersin.org/articles/10.3389/fcimb.2023.1195803/full
Author Ana Barbosa, Sónia Miranda,Nuno F. Azevedo, Laura Cerqueira, Andreia S. Azevedo

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current22:30, 23 October 2023Thumbnail for version as of 22:30, 23 October 20231,417 × 1,075 (240 KB)Ozzie10aaaa (talk | contribs)Uploaded a work by Ana Barbosa, Sónia Miranda,Nuno F. Azevedo, Laura Cerqueira, Andreia S. Azevedo from https://www.frontiersin.org/articles/10.3389/fcimb.2023.1195803/full with UploadWizard

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