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Source: Front. Cell. Infect. Microbiol., 07 November 2012 | doi:10.3389/fcimb.2012.00133 Comparative genomics and stx phage characterization of LEE-negative Shiga toxin-producing Escherichia coli Susan R. Steyert, Jason W. Sahl, Claire M. Fraser, Louise D. Teel Flemming Scheutz and David A. Rasko

Figure 5. Gene organization flanking the stx genes and Q gene phylogeny for the stx phages in the LEE-negative STEC isolates and LEE-positive O157:H7 EDL933. Gene organization comparisons are shown for (A) stx1-encoding phages and (B) stx2-encoding phages. The colors correspond to the following gene designations: gray, rusA; yellow, Q; orange, DNA methylase; pink, tRNA genes; red, stxAB; green, yjhS; blue, lysis S, and endolysin genes; white, all other genes, predominantly encoding hypothetical proteins. A cluster diagram based on the Q gene sequences was determined (C) and primers (Table 2) were designed to be specific for each cluster according to the colors: Q1 green, Q2 purple, Q3 turquoise, Q4 blue, Q5 magenta, Q6a orange, and Q6b red. Clusters circled by a solid black line denote a high level of stx induction, gray circles denote intermediate level induction, and broken lines denote lack of induction.
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Author Phylogeny Figures

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