File:Expression of extraembryonic ectoderm and ectoplacental cone markers in Cul4b-null embryos.png

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Fig 6. Expression of extraembryonic ectoderm and ectoplacental cone markers in Cul4b-null embryos.

Transcripts of Bmp4 (A-D), Hand1 (E-H), and Mash2 (I-L) were detected in Cul4b+/Y and Cul4bΔ/Y embryos in whole-mount RNA in situ hybridization experiments. At E6.5 and E7.5, the transcript levels of the extraembryonic ectoderm marker Bmp4 and the ectoplacental cone marker Hand1 cannot be discriminated between Cul4b+/Y and Cul4bΔ/Y embryos (A-H). The expression levels of the extraembryonic ectoderm and ectoplacental cone marker Mash2 were not distinct between Cul4b+/Y and Cul4bΔ/Y embryos at E6.5 (I, J). A severe reduction in the chorionic ectoderm in Cul4bΔ/Y embryos was observed compared to that in Cul4b+/Y embryos at E7.5 (K, L). All embryos were generated from Cul4b+/Δ females mated with Cul4b+/Y WT males or Cul4blox/Y;Prm1-Cre males. CE: chorionic ectoderm. Scale bar: 200 μm.
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Source https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0219221 Chen C-Y, Yu I-S, Pai C-H, Lin C-Y, Lin S-R, Chen Y-T, et al. (2019) Embryonic Cul4b is important for epiblast growth and location of primitive streak layer cells. PLoS ONE 14(7): e0219221. https://doi.org/10.1371/journal.pone.0219221
Author Chen C-Y, Yu I-S, Pai C-H, Lin C-Y, Lin S-R, Chen Y-T, et al.
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current19:55, 4 September 2024Thumbnail for version as of 19:55, 4 September 20242,220 × 1,397 (1.74 MB)Rasbak (talk | contribs){{Information |description=Fig 6. Expression of extraembryonic ectoderm and ectoplacental cone markers in Cul4b-null embryos.<br> Transcripts of Bmp4 (A-D), Hand1 (E-H), and Mash2 (I-L) were detected in Cul4b+/Y and Cul4bΔ/Y embryos in whole-mount RNA in situ hybridization experiments. At E6.5 and E7.5, the transcript levels of the extraembryonic ectoderm marker Bmp4 and the ectoplacental cone marker Hand1 cannot be discriminated between Cul4b+/Y and Cul4bΔ/Y embryos (A-H). The expression lev...

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