File:Ectopic-A-lattice-seams-destabilize-microtubules-ncomms4094-s5.ogv
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Ectopic-A-lattice-seams-destabilize-microtubules-ncomms4094-s5.ogv (Ogg Theora video file, length 20 s, 93 × 51 pixels, 345 kbps, file size: 843 KB)
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English: Supplementary Movie 4 A-lattice enriched MT seeds (green) were co-assembled from pig brain tubulin labelled with Alexa-488, Mal3FL and GMPCPP. Seeds were attached to flow cells coated with anti-Alexa488 antibodies. Flow cells were flushed with buffer to remove Mal3 and unbound MTs. Buffer containing GTP and unlabelled S. pombe single isoform tubulin (grey) was added and MT dynamics observed using timelapse epifluorescence (for seeds) and darkfield (for dynamic unlabelled S. pombe MTs) microscopy. MTs grown from the A-lattice enriched seeds had frequent episodes of transient short MT growth, which we have named piku-piku or MT dithering (blue label). Time is in minutes. Scale bar is 5 μm. |
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| Source | Video file from Katsuki M, Drummond D, Cross R (2014). "Ectopic A-lattice seams destabilize microtubules". Nature Communications. DOI:10.1038/ncomms4094. PMID 24463734. PMC: 3921467. | ||
| Author | Katsuki M, Drummond D, Cross R | ||
| Permission (Reusing this file) |
  This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 00:37, 3 November 2016 | 20 s, 93 × 51 (843 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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