File:DAAM1-Is-a-Formin-Required-for-Centrosome-Re-Orientation-during-Cell-Migration-pone.0013064.s004.ogv
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[edit]DescriptionDAAM1-Is-a-Formin-Required-for-Centrosome-Re-Orientation-during-Cell-Migration-pone.0013064.s004.ogv |
English: Migrational behavior of COS-7 cells upon DAAM1 knockdown. Control siRNA treated cells (Movies S1) or si2318 siRNA treated cells (Movie S2) were subjected to scratch wounding and analysis by phase contrast microscopy two days post-transfection. Images were acquired at 15 min intervals over 12 hours using a Zeiss Axiovert 135 M Inverted microscope equipped with a 10x/0.25 Plan-APROCHROMAT lens. Images were processed using Image-Pro Plus software. The nuclei of 6 cells were tracked in every frame using ImageJ software and the resultant paths are shown as colored lines. |
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Source | Movies S1 from Ang S, Zhao Z, Lim L, Manser E (2010). "DAAM1 Is a Formin Required for Centrosome Re-Orientation during Cell Migration". PLOS ONE. DOI:10.1371/journal.pone.0013064. PMID 20927366. PMC: 2947498. | ||
Author | Ang S, Zhao Z, Lim L, Manser E | ||
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current | 10:25, 31 October 2012 | 8.0 s, 1,036 × 684 (3.83 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Ang S, Zhao Z, Lim L, Manser E |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Migrational behavior of COS-7 cells upon DAAM1 knockdown. Control siRNA treated cells (Movies S1) or si2318 siRNA treated cells (Movie S2) were subjected to scratch wounding and analysis by phase contrast microscopy two days post-transfection. Images were acquired at 15 min intervals over 12 hours using a Zeiss Axiovert 135 M Inverted microscope equipped with a 10x/0.25 Plan-APROCHROMAT lens. Images were processed using Image-Pro Plus software. The nuclei of 6 cells were tracked in every frame using ImageJ software and the resultant paths are shown as colored lines. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010 |