File:Culture vials of Drosophila melanogaster in Unifei.jpg
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Português: Potes de plástico e vidro para criação de Drosophila melanogaster (Diptera; Drosophilidae), ou mosca-da-fruta, para fins didáticos.
Cada pote possui um meio de cultura para alimentação das moscas. Foram utilizados gelatina incolor, água, açúcar, farinha de trigo, nipagim (metilparabeno, fungicida) e fermento biológico. Os ingredientes foram misturados ao fogo até a mistura engrossar. Cada pote recebeu um pouco do meio de cultura e um filtro de papel, para a colocação de ovos das moscas, e foi tampado com um pedaço de pano presos com elástico, para permitir passagem do ar. Em cada pote foram colocados espécimes de D. melanogaster das seguintes linhagens: selvagem, taxi, ebony, taxi ebony, white, e vestigial. Cada linhagem com exceção da selvagem possuem uma ou mais mutações que afetam o seu fenótipo. As moscas se alimentam do meio de cultura, colocam ovos no filtro de papel e nas bordas dos potes, e se reproduzem. Em poucos dias é possível observar ovos, larvas, pupas e adultos dentro dos potes. A criação de D. melanogaster é muito utilizada por suas aplicações em genética, embriologia e diversas áreas do conhecimento, e pode ser utilizada durante as aulas para análise do ciclo de vida dos insetos e para estudo na prática das Leis de Mendel, uma vez que as características são herdáveis e podem ser observadas a olho nu. As linhagens de D. melanogaster foram obtidas do Laboratório de Drosophila da Universidade Federal de Viçosa (UFV) em Viçosa - MG, e foram doadas para a Universidade Federal de Itajubá (Unifei) em Itajubá - MG, Brasil.English: Plastic and glass vials used for creation of Drosophila melanogaster (Diptera; Drosophilidae), the fruit fly, for didactic purposes.
The ingredients used for the culture medium were tasteless gelatin, water, sugar, flour, methylparaben (fungicide) and biological yeast. The ingredients were heated until boiling. Each jar received some culture medium and a paper filter, for the flies to lay their eggs, and was sealed with a piece of cloth and a rubber band, to allow for the passage of air. Each jar received one of the following lineages of D. melanogaster: wild, taxi, ebony, taxi ebony, white, and vestigial. With the exception of wild, all lineages have one or more mutations that affect the phenotype. Flies feed of the medium, lay eggs on the paper filter and sides of the jars, and reproduce. Within few days it is possible to see eggs, larvae, pupae and adults inside. The creation of D. melanogaster is widely spread for its small cost and its applications in genetics, embriology and other fields of knowledge, and can be used for analysis of the life cycle of insects and for studying Mendel Laws, since the characteristics and inheritable and vsible to the naked eye. The lineages were obtained from Drosophila Laboratory of the Federal University of Viçosa (UFV) in Viçosa - MG, and samples were donated to the Federal University of Itajubá (Unifei) in Itajubá - MG, Brazil.English: Plastic and glass vials used for creation of Drosophila melanogaster (Diptera; Drosophilidae), the fruit fly, for didactic purposes in Federal University of Itajubá, Brazil. |
| Date | |
| Source | Own work |
| Author | Mateus S. Figueiredo |
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| current | 20:14, 12 November 2017 | | 4,437 × 2,404 (3.17 MB) | Mateussf (talk | contribs) | User created page with UploadWizard |
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