File:Contributions from the Botanical Laboratory, vol. 12 (1934) (20500341530).jpg

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Title: Contributions from the Botanical Laboratory, vol. 12
Identifier: contributionsfro12univ (find matches)
Year: 1934 (1930s)
Authors: University of Pennsylvania. Botanical Laboratory; University of Pennsylvania. Morris Arboretum
Subjects: Botany; Botany
Publisher: Philadelphia : (s. n. )
Contributing Library: Penn State University
Digitizing Sponsor: Lyrasis Members and Sloan Foundation

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28 STAIN TFXHNOLOGY
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V* IK Descutption of Plate I iron Tvlipifera infected-'^'^ ^'^^J^l^^^ -^ ,,tânin.ith no counterstam^Figs. I orseilUn BB and crystal violet, ^'g- »'^^J â ^ o.65; Fig. 2 with a Spencer andSweretaUenwUhaBauscha^^^^ iâion fluorite. n. a. t.S. 11at^🅰a LI Xtcl; was used on all photomicrographs. K d in Conanfs Modified showing shrunken host cells and hyphaewh.ch "^fi ed rhX^sUuLres. Magnification: XSOO. *J^ THE FIXING AND STAINING OF ROOT TIPS Results » ahSn*? 7' <^«ntaining a strong acid which seemingly penetrates t^^:^iZ!^LtT'^^^^^ â '^^^^^^ hollow^t^ucturt bu renorreH hi 7 ^f ^f'"^ ^"*'^'^ ^^ ^"*=^ combinations has been reported by Zirkle m several of his papers dealing with fixation Tt Z descnbed above but the walls of the hyphae as well as the ceU walls o lf«rbu7the dT:' '°? t""'^" ^^^^- '^- F°--Idehyde aln I ^^^:^iz:i:t: HTnt=rii:r ^Tr^ whicl dei^^LX^^^ " ""^ ""'"' â¢^^^^ ^'^^^ ^"^-^ -^stances rsupoorted bv « ^^^^at.on of the fluid. This observation 1 mT i 1 ^ comparison between specimens preserved in a for maldehyde fixative containing the acid .salt, ferric chloride and thos" specimens preserved in a fixative containing the relatively basic sub stances, phenol, resorcinol, or pyrogallol (Fig. 3). A mixtL o feTrt bifed'^th cf "â¢'" ^."'^^'r' ^^"^ "^^'"^ â¢^^-' ^"t -hen com- bined with chromic acid (Cohen, 1934) it was not as satisfactory as when conibmed either with formaldehyde alone or with formaldehyde mixed with chromic sulfate in preserving the cell walls and the Jphae ot the fungus (Figs. 2 and 6). o-pnie ^ tt\ T!^ '" ' 'T" ^""^ ""'* fo^^'IdeMe showing the fixation of the cell walls of the host tissue and the intimate association of the intracellular hyphae The t" cells on the nght show the hyphae partly digested. Magnification: XS^ Figure 3. Fixed in ferric chloride and formaldehyde. The heavily colored spherical "'^^^^iX:^:::^:^ ^--^^ -^ ^â¢-- ^^^ -topiasmio Figure 5. Fixed in chromic sulfate, formaldehyde, copper hydroxide showing the asso t^M" ^'^"^ "' '""^ """ "^^"^ ""â¢^ ^'â¢^' -"^ °f ^'"^ fungus Magnml Figure 6. Fixed in chromic sulfate, formaldehyde, salicylic acid showing the fine preser- itZuZo"^ "'"^ °' * '""^' "â¢^ ^"^ '=^" -"^ °f ^^ hoft tissue. mT;::.
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University of Pennsylvania. Botanical Laboratory;

University of Pennsylvania. Morris Arboretum
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