File:Contributions from the Botanical Laboratory, vol. 12 (1934) (20067373373).jpg

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Title: Contributions from the Botanical Laboratory, vol. 12
Identifier: contributionsfro12univ (find matches)
Year: 1934 (1930s)
Authors: University of Pennsylvania. Botanical Laboratory; University of Pennsylvania. Morris Arboretum
Subjects: Botany; Botany
Publisher: Philadelphia : (s. n. )
Contributing Library: Penn State University
Digitizing Sponsor: Lyrasis Members and Sloan Foundation

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28 STAIN TEC HNOLOGY â ^^v^"*"*'^ M
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Debchiption of Plate 1 iron Wvifera infecte,' -^^^'^ f^'"^ ^^^j ^ ,,(,«ââ .ith no counter.tain^K.g- t orseillin BB and crystal v,olet. ^'g- ^-^^^^J^; . ,,rv, n. a. 0.G5; Fig. 2 with a Spencer -r;-^^"te;. Fi^s'ttaTd ':! a^Zei. oi. i.â⢠fluoHte, â⢠a. 1.3. fi^^Z Hb XC ocnla. .a. used on a,l photomicrographs. C ^. -a in Conant. Mo.Jed .o.ng .ru U. Host ceâs and hyphae .h.ch tlfixed as hollow structures. Magnification. X300. Ml "'1* L. <<'JI I ^j ^ THE FIXING AND STAINING OF ROOT TIPS 29 Ri:suLTs usenii, but the addition of other reuL'ents imt>rr.,.«= .f c i- ⢠Sub.stances which increase tl,e J! '^ "*' 'mproves its fixation image, dehvde hnt w inl '"'^'^';,»^ I*"'"" concentration of the formal- dehyde but which apparently do not penetrate the .s,,ecimen ahead of Ts tforteThv " concentration of the fluid. This oh.servation supported by a comparison between specimens j,re.served in a for maldehyde fixative containing the acid .salt, ferric diloride andlhose irslZrr' '" ' f"- r^'"â¢"^' relative,; w'r â¢stances, phenol, resorcinol, or pyrogallol (Fig. 3). A mixture of ferric Salicylic acid in all fluids tried gave useful images, but when com bined with chromic acid (Cohen, 1934) it was not a satis actorH when combined either with formaldehyde alone or with forma^Sthyd mixed with chromic sulfate in preserving the cell walls and the hyphae of the fungus (Figs. 2 and 6). ^' Tnl\ T!^ '" ' i 'f "'"' ""'^ f°â¢a'dehyde showing the fixation of the cell w,lls of he host tissue and the intimate association of the intracellular hvphae The ul cells on the nght show the hyphae partly digested. Magnification: X3oi. Figure 3. Fixed in ferric chloride and formaldehyde. The heavily colored spherical ismic Figure 4 Fixed in chromic sulfate, formaldehyde, picric acid showing the cytoplas contents of the hyphae. Magnification: XG60. fe i"e cy toplas ^'Tr 'â ^;',T '"Chromic sulfate, formaldehyde, copper hydroxide showing the asso- L; C "â '"'^ "' '" -'"' ^"^ â '^P""' -"^ °f "- f'"Kus. Magnml- Figure 6. Fixed in chromic sulfate, formaldehyde, salicylic aci.l showing the fine preser- czZo"" ^""^"' *'^ "^"' '^^ -" «'>"^ "f t"^ â¢>-' tissue.x;::. INTENTIONAL SECOND EXPOSURE
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University of Pennsylvania. Botanical Laboratory;

University of Pennsylvania. Morris Arboretum
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