File:Cell-type specificity of TIP-YFP expression in the root axis cropped.jpg
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DescriptionCell-type specificity of TIP-YFP expression in the root axis cropped.jpg |
English: Cell-type specificity of TIP-YFP expression in the root axis. 8-day old roots from the indicated transgenic lines were excised, stained with propidium iodide for 2 min and visualised by CLSM. Stacks of 80 optical z sections (1 μm step-size) were collected from root axes at the differentiation zone. The images show representative results for each construct. A to F: for each panel, the top section shows a single xy optical section, and the bottom section shows the xz projection of the whole image stack, revealing the cross section of the root axis. The signals from YFP fluorescence (green) and propidium iodide fluorescence (red) are merged. G and H: the YFP fluorescence trace from representative image stacks for the indicated transgenic lines was reconstructed, segmented and rendered in 3D with Mimics 12.1. The different tissues are colour-coded as follows: brown, epidermis; red, cortex; blue, endodermis; green, pericycle. Ep, epidermis; c, cortex; e, endodermis; p, pericycle; x, xylem. Scale bar: 20 μm. |
Date | (UTC) |
Source | Gattolin et al. In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots, BMC Plant Biology 2009 9:133 doi:10.1186/1471-2229-9-133 |
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This is a retouched picture, which means that it has been digitally altered from its original version. Modifications: cropped. The original can be viewed here: Cell-type specificity of TIP-YFP expression in the root axis.jpg: . Modifications made by CopperKettle.
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[edit]This image is a derivative work of the following images:
- File:Cell-type_specificity_of_TIP-YFP_expression_in_the_root_axis.jpg licensed with Cc-by-2.0
- 2009-12-27T08:41:46Z CopperKettle 1200x2007 (567022 Bytes) {{Information |Description={{en|1=Cell-type specificity of TIP-YFP expression in the root axis. 8-day old roots from the indicated transgenic lines were excised, stained with propidium iodide for 2 min and visualised by CLSM.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 19:42, 31 July 2012 | 1,050 × 565 (120 KB) | Daniel Mietchen (talk | contribs) | cropped; removed fonts | |
08:52, 27 December 2009 | 1,061 × 575 (149 KB) | CopperKettle (talk | contribs) | {{Information |Description={{en|1=Cell-type specificity of TIP-YFP expression in the root axis. 8-day old roots from the indicated transgenic lines were excised, stained with propidium iodide for 2 min and visualised by CLSM. Stacks of 80 optical z sectio |
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