File:Approach to generate 3D models of the developing ovary.jpg
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Figure 2—figure supplement 1. Approach to generate 3D models of the developing ovary. (A) Schematic depicting the basic pipeline for producing 3D images of the fetal mouse ovary using iDISCO+CUBIC tissue clearing and lightsheet microscopy. (B) Approach for generating 3D models in Imaris software using isosurface segmentation of lightsheet images for each of the main markers used in this study. The left column shows the 3D maximum intensity projection view of the raw lightsheet Z-stack of an ovary/mesonephros complex E16.5 immunostained for FOXL2 (cyan) and PAX8 (red), and counterstained with Hoechst nuclear dye (grayscale); the middle column shows the process of isosurface segmentation in Imaris and the right column shows the final 3D surfaces used in the figures throughout the manuscript. MD, Mullerian duct; Mn, mesonephros; RO, rete ovarii. Scale bars, 100 μm. |
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| Source | https://elifesciences.org/articles/81088 Jennifer McKey, Dilara N. Anbarci, Corey Bunce, Alejandra E. Ontiveros, Richard R. Behringer, Blanche Capel Integration of mouse ovary morphogenesis with developmental dynamics of the oviduct, ovarian ligaments, and rete ovarii, bioRxiv 2021.05.21.445181; doi: https://doi.org/10.1101/2021.05.21.445181 |
| Author | Jennifer McKey, Dilara N. Anbarci, Corey Bunce, Alejandra E. Ontiveros, Richard R. Behringer, Blanche Capel |
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© 2022, McKey et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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| current | 10:04, 6 August 2024 | | 3,561 × 4,828 (1.42 MB) | Rasbak (talk | contribs) | {{Information |description=Figure 2—figure supplement 1. Approach to generate 3D models of the developing ovary. (A) Schematic depicting the basic pipeline for producing 3D images of the fetal mouse ovary using iDISCO+CUBIC tissue clearing and lightsheet microscopy. (B) Approach for generating 3D models in Imaris software using isosurface segmentation of lightsheet images for each of the main markers used in this study. The left column shows the 3D maximum intensity projection view of the ra... |
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