File:Applied immunology - the practical application of sera and bacterins prophylactically, diagnostically, and therapeutically (1916) (14768717402).jpg

Identifier: appliedimmunolog00thom (find matches)
Title: Applied immunology : the practical application of sera and bacterins prophylactically, diagnostically, and therapeutically
Year: 1916 (1910s)
Authors: Thomas, Benjamin Abraham, 1878- Ivy, Robert Henry, 1881-
Subjects: Serotherapy Immunity Immunization, Passive
Publisher: Philadelphia : J.B. Lippincott
Contributing Library: Francis A. Countway Library of Medicine
Digitizing Sponsor: Open Knowledge Commons and Harvard Medical School

View Book Page: Book Viewer
About This Book: Catalog Entry
View All Images: All Images From Book
Click here to view book online to see this illustration in context in a browseable online version of this book.

Text Appearing Before Image:
n byserum and antigen alone, and also to make up forthe complement lost during incubation, and at thesame time use in the test proper not more than onehemolytic unit. Previous to titration of comple-ment with hemolytic amboceptor and corpuscles, thecomplement is first incubated with one dose of antigenand 0.1 c.c. of a mixture of several non-syphiliticsera. This is done for the reason that a slight amountof complement is fixed under these conditions by theserum and antigen, and we are thus sure of allowingfor this slight non-specific fixation in the test itself.Different sera present variations in the amount ofcomplement they are capable of absorbing in this FIXATION OF COMPLEMENT 119 manner, but these variations are usually so slightthat for practical purposes they can be ignored,particularly if a pooled serum from several non-syphilitic cases be used. The technic of titrationof complement is carried out as follows: Into eachof a series of six tubes is placed 0.2, 0.3, 0.4, 0.5, 0.6,
Text Appearing After Image:
Fig. 13.—Titration of complement. With the complement in each tube are placed0.1 c.c. of non-syphilitic serum and 1 dose of antigen. The tubes contain graduallyincreasing amounts of complement, ranging from 0.2 c.c. (dilution 1-10) to 0.8 c.c. 0.7 and 0.8 c.c. of 1-10 dilution of guinea-pig serum,together with 0.1 c.c. of pooled non-syphilitic serumpreviously inactivated by heating at 56° C. for half anhour, and the dose of antigen used in the test (Fig.13). Sufficient salt solution is then added to bring thevolume up to 2 c.c. The tubes are shaken and incu- 120 APPLIED IMMUNOLOGY bated for one hour at 37° C, after which 1 c.c. of1-1000 haemolytie amboceptor and 1 c.c. of 5 per cent,suspension of sheeps corpuscles are added to eachtube. After a further incubation of one hour, the de-gree of haemolysis that has taken place in the differenttubes is noted. Usually complete haemolysis has oc-curred in all the tubes containing 0.5 c.c. and more ofdiluted complement. The 0.5 c.c. tube i

Note About Images