File:Applied immunology - the practical application of sera and bacterins prophylactically, diagnostically, and therapeutically (1916) (14582410078).jpg

Identifier: appliedimmunolog00thom (find matches)
Title: Applied immunology : the practical application of sera and bacterins prophylactically, diagnostically, and therapeutically
Year: 1916 (1910s)
Authors: Thomas, Benjamin Abraham, 1878- Ivy, Robert Henry, 1881-
Subjects: Serotherapy Immunity Immunization, Passive
Publisher: Philadelphia : J.B. Lippincott
Contributing Library: Francis A. Countway Library of Medicine
Digitizing Sponsor: Open Knowledge Commons and Harvard Medical School

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uspension of sheeps corpuscles prepared asdescribed above may be injected into the rabbit eitherintraperitoneally or intravenously. Since much largeramounts of suspension and a longer time for immu-nization are required by the former method, we havediscarded it entirely in favor of the intravenousmethod. The latter is just as simple, and ifproper precautions against infection and injectionof air be taken, is quite safe. For the first injectionabout 1 c.c. of the 10 per cent, corpuscle suspension isdrawn up into an all-glass hypodermic syringe of 2c.c. capacity and fitted with the usual fine needle; thesmaller the needle the better. The marginal vein ofthe rabbits ear is made prominent by wiping it with FIXATION OF COMPLEMENT 107 xylol, and the suspension slowly injected into itthrough the skin in the direction of the circulation(Fig. 8). Usually, to obtain a sufficiently powerfulhemolytic amboceptor, three injections at intervals of3 or 4 days are required. The second injection should
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Fig. 8.—Showing method of intravenous injection or immunization of rabbit with10 per cent, suspension of sheeps blood-corpuscles for the production of hemolyticamboceptor. consist of 1.5 c.c. and the third of 2 c.c. of the sus-pension. Three or 4 days after the third injection,about 2 c.c. of the rabbits blood is drawn from the earby puncturing a vein, and the serum tested for itshemolytic power against sheeps corpuscles. Thus in 108 APPLIED IMMUNOLOGY ten days, or two weeks at the most, we are able to pre-pare a powerful hemolytic amboceptor, which re-quires four or five weeks by the intraperitonealmethod. Titration of Hcemolytic Amboceptor.—For use inthe Wassermann test we aim to produce a hemolyticamboceptor of such strength that 1 c.c. of not less thana 1-2000 dilution of rabbits serum will hsemolyze 1c.c. of a 5 per cent, suspension of sheeps corpusclesin the presence of 0.1 c.c. of fresh guinea-pig serumused as complement. Usually, by the intravenousmethod described, the rab

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