File:Annual report (1938) (18243257549).jpg

Title: Annual report
Identifier: annualreport19381943ento (find matches)
Year: 1872 (1870s)
Authors: Entomological Society of Ontario; Ontario. Dept. of Agriculture
Subjects: Entomological Society of Ontario; Insect pests; Insects
Publisher: Toronto, The Society
Contributing Library: Smithsonian Libraries
Digitizing Sponsor: Biodiversity Heritage Library

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Plate 1.—Aging Cage, Egg-laying Cage. Type of cage used for oviposition and for aging of flies. Separate items (left to right), (1) Wooden frame and bottom of the cage, (2) Cage as used for ag- ing flies, (3) As used for oviposition, (4) Tray for culture medium in egg-laying cage. Maturing Cage.—The culture medium with accumulated larvae and eggs is taken from the tray and put on the "floor" at the back end of a flat sided gum jar which is kept lying on its side. If necessary, a little fresh culture medium is added. The front half of the "floor" is then cover- ed with clean, dry, wheat bran, to a depth of approximately y2 or yA of an inch. This absorbs excess moisture and also provides a favourable medium for pupation. The opening is covered with a square of cotton held in place by means of an elastic band. Both the egg-laying cages and the maturing cages are kept in the same constant temperature cham- ber, at a temperature of approximately 70 deg. F. The relative humidity in this cage varies somewhat but is approximately 70 per cent most of the time. Spraying The Flies Aging Cage.—As the flies appear in the maturing cage, they are transferred at intervals of either one or two days, to a third cage in which they are kept until ready for spraying. A fresh cage is used, of course, for all flies transferred on any one day. This cage is constructed the same as the egg-laying cage. In fact, the same cage may be used for either purpose. When used for aging, the food and moisture requirements of the flies are supplied by means of a long cotton-wool plug soaked with a 3 per cent honey solution. This is done by filling a long vial (15 m.m. x 100 m.m.) with the honey solution and then stoppering it with a wad of cotton batting. The vial is then turned upside down and inserted through a close fitting hole in the cloth top so that the honey solution will seep down and replace that used by the flies and by evaporation. Two of these feeding vials are generally inserted in the roof of each cage. Rub- ber bands placed about the vials prevent them from dropping into the cage.

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