File:An-Intrinsically-Disordered-Region-of-the-Acetyltransferase-p300-with-Similarity-to-Prion-Like-pone.0048243.s007.ogv

English: Dynamics of aggresomes in the presence of RFP-p300-PSPD. Cells were transfected with the vector expressing RFP-p300-PSPD, plated onto Lab-Tek II Chambered coverglasses System dish, and then treated with 5 µM MG132. The dishes were placed in a temperature-controlled stage at 37°C on an inverted microscope (Nikon Eclipse TE300) in a 5% CO2 atmosphere, and subjected to time-lapse video microscopy. Images were collected using a 40x lens, with a filter set for DsRED (excitation 545 nm, dichroic 570 nm, emission 620 nm), and a Hamamatsu Orca-ER, monochrome cooled CCD camera. Images were taken at 15 min intervals for 16 h with MetaMorph Imaging system, and were then assembled into QuickTime movies.