File:Aliran Proses Untuk Pembuatan Insulin.png
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[edit]DescriptionAliran Proses Untuk Pembuatan Insulin.png |
English: 8 different *major steps of producing insulins starting from using human pancreas cells until the insulin itself.
Step 1: The insulin gene is a protein consisting of two separate chains of amino acids, an A above a B chain, that are held together with bonds. Both insulin genes is extracted from stem cells of healthy human pancreas using restriction enzymes. Step 2: Using same restriction enzymes, plasmids are cut open for the insulin genes to attach to. First, add a mixture of lysozome that digest the outer layer of the cell wall, second adding a detergent mixture that separates the fatty cell wall membrane. Third, adding cyanogen bromide, a reagent that splits insulin chains from the plasmid chains Step 7: The two insulin gene chains are then mixed together and joined by disulfide bonds through the reduction-oxidation reaction. An oxidizing agent (a material that causes oxidization or the transfer of an electron) is added. |
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Source | Own work |
Author | Encik Tekateki |
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current | 08:20, 26 December 2022 | ![]() | 1,456 × 746 (57 KB) | Encik Tekateki (talk | contribs) | Uploaded own work with UploadWizard |
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