File:Actin dynamics and buccopharyngeal perforation of Xenopus laevis.png

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Description Figure 6. Actin dynamics and buccopharyngeal perforation. A-G) Actin dynamics revealed by phalloidin labeling during buccopharyngeal membrane perforation. A) Shows a frontal view of the face at stage 39 (representative from 30 embryos in 4 biological replicates). B) The buccopharyngeal membrane (BPM) at stage 37 when the mouth shape is round. C) The buccopharyngeal membrane at stage 39 just prior to perforation. Note the puncta spots and accumulation of F-actin (MCCs) (white arrows). D) The buccopharyngeal membrane (BPM) during perforation shows accumulation of F-actin surrounding the holes. E-G) The buccopharyngeal membranes control morphants (E), jak2 morphants (F), and myod morphants (G). H-J) Images of representative embryos after treating with cytochalasin D (1uM) and Rockout (100uM) from stages 37-40. K) Quantification of buccopharyngeal membrane rupture in embryos exposed to actin inhibitors shown in H-J' (n= 60, 3 biological replicates). Xenopus laevis. nos=nostril, MO=morpholino, BPM=buccopharyngeal membrane.
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Source https://www.researchgate.net/publication/369979586_Jak2_and_Jaw_Muscles_Are_Required_for_Mouth_Development Dickinson, A.J.G. Jak2 and Jaw Muscles Are Required for Buccopharyngeal Membrane Perforation during Mouth Development. J. Dev. Biol. 2023, 11, 24.
Author Amanda J.G. Dickinson
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current10:38, 26 June 2024Thumbnail for version as of 10:38, 26 June 2024982 × 933 (1.04 MB)Rasbak (talk | contribs){{Information |description=Figure 6. Actin dynamics and buccopharyngeal perforation. A-G) Actin dynamics revealed by phalloidin labeling during buccopharyngeal membrane perforation. A) Shows a frontal view of the face at stage 39 (representative from 30 embryos in 4 biological replicates). B) The buccopharyngeal membrane at stage 37 when the mouth shape is round. C) The buccopharyngeal membrane at stage 39 just prior to perforation. Note the puncta spots and accumulation of F-actin (white arr...

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