File:41564 2023 1503 Fig6 HTML.jpg

English: a, Total FDG activity (lung inflammation) relative to Mtb challenge, measured by PET/CT imaging. Lines indicate individual animals. b, Number of lung granulomas relative to Mtb challenge. Animals with granuloma numbers >100 are indicated as too numerous to count (tntc). At 4 and 8 weeks, granulomas were counted by CT, whereas at 12 weeks, granulomas were counted by gross pathology. c, Three-dimensional renderings of PET/CT images of individual animals taken at necropsy. d–f, Lung inflammation (d), overall TB pathology (e) and total Mtb burden (thoracic CFU) (f) at necropsy. Each point indicates an individual animal and horizontal bars indicate group medians (Unvax, n = 8; IV BCG, n = 7; SIV/Unvax, n = 11; SIV/IV BCG, n = 12). Kruskal–Wallis tests were performed with Dunn’s multiple comparisons between SIV-naive, vaccinated and unvaccinated groups (dark-blue circles and light-blue squares, respectively), and SIV+, vaccinated and unvaccinated groups (red, up-pointing triangle and gold, down-pointing triangle, respectively). P values are shown. All statistical tests were two-sided.